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产品描述：人Thy-1膜糖蛋白(THY1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的THY1含量。THY1是一个重要靶点。它在细胞黏附、信号传导等方面发挥关键作用。研究发现其与神经系统发育、肿瘤进展等多种生理病理过程相关。目前围绕它探究调控机制，如信号通路和相关分子互作，以便开发针对性治疗手段。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于神经生物学、免疫学及纤维化疾病机制研究，例如探索THY1在神经退行性病变模型中的表达动态、评估组织修复过程中成纤维细胞的活化状态，或分析免疫细胞表面标记物在炎症微环境中的调控作用本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD7 ELISA Kit; CD90 ELISA Kit; CD90 antigen ELISA Kit; CDw90 ELISA Kit; FLJ33325 ELISA Kit; MGC128895 ELISA Kit; T25 ELISA Kit; Theta antigen ELISA Kit; Thy 1 ELISA Kit; Thy 1 cell surface antigen ELISA Kit; Thy 1 membrane glycoprotein ELISA Kit; Thy 1 T cell antigen ELISA Kit; Thy 1.2 ELISA Kit; Thy-1 antigen ELISA Kit; Thy-1 membrane glycoprotein ELISA Kit; Thy1 ELISA Kit; Thy1 antigen ELISA Kit; Thy1 T cell antigen ELISA Kit; Thy1.1 ELISA Kit; Thy1.2 ELISA Kit; THY1_HUMAN ELISA Kit; Thymus cell antigen 1, theta ELISA Kit
缩写：THY1
Uniprot No.：P04216
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human THY1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:40 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:80 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:160 | Average % | 96 | ||||
| Range % | 93-99 | |||||
回收率：
| The recovery of human THY1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-89 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.6082.6582.6332.449202.0032.1302.0671.883101.3961.3751.3861.20250.8720.8680.8700.6862.50.5050.5360.5210.3371.250.3120.3320.3220.1380.6250.2610.2780.2700.08600.1810.1860.184 | ||||||
货期：3-5 working days
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