首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血小板生成素(TPO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的THPO含量。THPO即血小板生成素，它是调节血小板生成的关键因子。与巨核细胞及血小板表面的受体结合，促进巨核细胞增殖、分化和成熟，从而增加血小板生成。在造血系统疾病治疗等领域有重要研究价值。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于体外研究TPO在血液系统疾病模型中的调控机制、药物干预效果评估或生理病理状态下的动态变化分析，为血液学、免疫学等基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C mpl ligand ELISA Kit; C-mpl ligand ELISA Kit; Megakaryocyte colony stimulating factor ELISA Kit; Megakaryocyte colony-stimulating factor ELISA Kit; Megakaryocyte growth and development factor ELISA Kit; Megakaryocyte stimulating factor ELISA Kit; MGC163194 ELISA Kit; MGDF ELISA Kit; MKCSF ELISA Kit; ML ELISA Kit; MPL ligand ELISA Kit; MPLLG ELISA Kit; Myeloproliferative leukemia virus oncogene ligand ELISA Kit; Prepro thrombopoietin ELISA Kit; THCYT1 ELISA Kit; THPO ELISA Kit; Thrombopoietin ELISA Kit; Thrombopoietin nirs variant 1 ELISA Kit; TPO ELISA Kit; TPO_HUMAN ELISA Kit
缩写：THPO
Uniprot No.：P40225
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of human TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-102 | ||||
| EDTA plasma (n=4) | 88 | 82-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.6642.5722.6182.4832002.0852.1782.1321.9971001.4891.3941.4421.307500.8220.8420.8320.697250.4330.4570.4450.31012.50.2930.2840.2890.1546.250.1960.1980.1970.06200.1340.1350.135 | ||||||
货期：3-5 working days
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