首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠可溶性血栓调节蛋白 (sTM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的THBD含量。THBD即血栓调节蛋白，其在维持血管内凝血与抗凝平衡、抗炎等方面有重要背景意义。研究机制上，它可与凝血酶结合，改变凝血酶性质，激活蛋白C抗凝系统，抑制凝血，还能减轻炎症反应，在血栓性疾病等研究中受关注。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于心血管疾病模型、血栓形成机制研究、内皮功能评估等科研领域，尤其可用于动脉粥样硬化、糖尿病血管病变或败血症等动物模型的生物标志物分析。为血管生物学、凝血病理机制及药物干预效果评估提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Thbd; Thrombomodulin; TM; Fetomodulin; CD antigen CD141
缩写：THBD
Uniprot No.：P15306
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse STM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 88 | |||||
| Range % | 81-92 | ||||||
| 1:10 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
| 1:20 | Average % | 86 | |||||
| Range % | 83-89 | ||||||
| 1:40 | Average % | 95 | |||||
| Range % | 91-99 | ||||||
回收率：
| The recovery of mouse STM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 84 | 81-86 | |||||
| EDTA plasma (n=4) | 89 | 85-93 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.6572.5032.5802.44751.7431.6981.7211.5882.50.9670.9970.9820.8491.250.5720.5910.5820.4490.6250.4160.4210.4190.2860.3120.2930.2860.2900.1570.1560.1940.1870.1910.05800.1320.1340.133 | |||||||
×
