| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CASP1; IL1BC; IL1BCE; Caspase-1; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45 |
| Entrez GeneID | 834 |
| WB Predicted band size | Calculated MW: 45 kDa; Observed MW: 45,20 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human Caspase-1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是3-4条关于Caspase 1抗体的参考文献(示例格式，非真实文献，仅供框架参考)：
---
1. **文献名称**: *The inflammasome: a molecular platform triggering activation of inflammatory caspases*
**作者**: Martinon F, et al.
**摘要**: 该研究通过Western blot和免疫沉淀实验验证Caspase 1抗体的特异性，揭示炎症小体激活Caspase 1的分子机制，证明其在IL-1β成熟中的关键作用。
2. **文献名称**: *Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion*
**作者**: Shi J, et al.
**摘要**: 利用Caspase 1抗体检测细胞焦亡过程中Caspase 1的活化，证实GSDMD是Caspase 1的下游切割靶点，驱动炎症因子释放。
3. **文献名称**: *Non-canonical inflammasome activation targets caspase-11*
**作者**: Kayagaki N, et al.
**摘要**: 通过Caspase 1抗体对比经典与非经典炎症小体通路，发现Caspase 1在革兰氏阴性菌感染中与Caspase 11的功能差异。
4. **文献名称**: *Caspase-1-dependent pore formation during pyroptosis*
**作者**: Bergsbaken T, et al.
**摘要**: 研究利用Caspase 1敲除模型及抗体，证明Caspase 1通过切割gasdermin蛋白诱导细胞膜孔形成，促进炎症反应。
---
*注：以上为模拟示例，实际文献需通过PubMed或Google Scholar检索关键词“Caspase 1 antibody”、“inflammasome”、“pyroptosis”获取。推荐参考领域权威期刊如《Nature Immunology》《Cell》《Immunity》。*
Caspase-1. also known as interleukin-1β-converting enzyme (ICE), is a cysteine-aspartic protease critical in mediating inflammatory responses and pyroptosis. It is synthesized as an inactive zymogen (pro-caspase-1) that undergoes autoproteolytic activation upon assembly of inflammasome complexes, such as NLRP3 or AIM2. in response to pathogens or cellular stress. Activated caspase-1 cleaves pro-inflammatory cytokines IL-1β and IL-18 into their mature forms, promoting inflammation, and processes gasdermin D to induce pyroptotic cell death. Dysregulation of caspase-1 is implicated in autoimmune disorders, neurodegenerative diseases, and infections.
Caspase-1 antibodies are essential tools for detecting and studying this enzyme in research. They are used in techniques like Western blotting, immunohistochemistry, and ELISA to identify caspase-1 expression, activation (via detection of cleaved subunits, p20/p10), or localization. Polyclonal or monoclonal antibodies targeting specific epitopes (e.g., pro-caspase-1. active fragments) are generated in hosts like rabbits, mice, or goats. Validated antibodies often undergo knockout cell line controls to confirm specificity. Researchers employ these antibodies to explore inflammasome signaling, disease mechanisms, or therapeutic targeting. Proper validation ensures reliable detection of caspase-1's dual roles in immunity and cell death pathways.
×
