首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠生长抑素(SS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SST含量。SST即生长抑素，可抑制多种激素分泌。其背景上，在人体内分泌调节等多生理过程中起重要作用。研究机制方面，主要是与生长抑素受体结合，调控细胞增殖、分化，在神经内分泌肿瘤等疾病诊疗研究中是重要靶点。试剂盒检测范围为4.7 pg/mL-300 pg/mL，适用于基础科研领域，如探究SS在代谢性疾病、神经退行性疾病或消化系统功能异常动物模型中的表达规律，也可用于评估药物干预或基因修饰对SS分泌通路的影响，为相关机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sst ELISA Kit; Smst ELISA Kit; Somatostatin [Cleaved into: Antrin; Somatostatin-28; Somatostatin-14; Neuronostatin ELISA Kit; NST)] ELISA Kit
缩写：SST
Uniprot No.：P60041
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：4.7 pg/mL-300 pg/mL
灵敏度：1.1 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-100 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of mouse SS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-99 | ||||
| EDTA plasma (n=4) | 94 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 3002.1231.9782.0511.940 1501.7391.6761.7081.597 751.3671.3011.3341.223 37.50.8940.8590.8770.766 18.750.6380.6270.6330.522 9.40.3550.3290.3420.231 4.70.2190.2110.2150.104 00.1120.1090.111 | ||||||
货期：3-5 working days
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