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产品描述：人3-羰基-5-α类固醇4脱氢酶1(SRD5A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SRD5A1含量。SRD5A1是一种重要靶点。它参与雄激素代谢，可将睾酮转化为活性更强的双氢睾酮。在脱发、良性前列腺增生等疾病进程中发挥作用。对其研究机制主要聚焦于开发抑制该靶点活性的药物，以调节体内雄激素水平，缓解相关病症。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于细胞生物学、分子机制研究及疾病模型构建等科研领域，如探究激素代谢通路调控、药物靶点筛选或SRD5A1在肿瘤微环境中的作用分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SRD5A1; 3-oxo-5-alpha-steroid 4-dehydrogenase 1; SR type 1; Steroid 5-alpha-reductase 1; S5AR 1
缩写：SRD5A1
Uniprot No.：P18405
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SRD5A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 95-106 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of human SRD5A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-93 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.0172.0632.0401.79551.4991.4721.4861.2412.51.0041.0641.0340.7891.250.7050.7420.7240.4790.6250.5010.5360.5190.2740.3120.4120.4380.4250.1800.1560.3470.3610.3540.10900.2410.2490.245 | ||||||
货期：3-5 working days
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