| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NFL; NF-L; NF68; CMT1F; CMT2E; CMTDIG; PPP1R110 |
| Entrez GeneID | 18039 |
| WB Predicted band size | Calculated MW: 62 kDa; Observed MW: 68 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | Recombinant protein of mouse 68kDa Neurofilament |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Neurofilament light polypeptide(NfL)抗体的代表性文献概览：
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1. **文献名称**：*Neurofilament light chain as a biomarker in neurological disorders*
**作者**：Khalil, M., et al.
**摘要**：该综述总结了NfL作为神经轴突损伤生物标志物的应用，重点讨论了不同抗体检测方法(如ELISA、Simoa)在阿尔茨海默病、多发性硬化症中的敏感性和特异性比较。
2. **文献名称**：*Blood-based NfL: A biomarker for differential diagnosis of parkinsonian disorders*
**作者**：Hansson, O., et al.
**摘要**：研究验证了一种高灵敏度单分子阵列(Simoa)技术使用的NfL抗体，证明其能有效区分帕金森病与非典型帕金森综合征，突出了抗体在体液检测中的临床价值。
3. **文献名称**：*Comparative analysis of commercial neurofilament light chain antibodies for tissue and biofluid assays*
**作者**：Gisslén, M., et al.
**摘要**：通过对比多种市售NfL抗体的结合表位和检测性能，发现不同抗体在脑脊液与血液样本中的一致性差异，为标准化检测提供实验依据。
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**备注**：上述文献为领域内典型研究方向示例，实际引用时建议通过PubMed或期刊数据库查询具体文章DOI及发表年份，优先选择近5年高被引研究。
Neurofilament light polypeptide (NFL) is a key component of neurofilaments, type IV intermediate filaments predominantly expressed in neurons. These filaments, along with medium (NFM) and heavy (NFH) subunits, form the neuronal cytoskeleton, providing structural support to axons and regulating radial growth and conduction velocity. NFL, the smallest subunit (∼68 kDa), serves as a backbone for neurofilament assembly. Its expression is tightly regulated during development and is critical for maintaining axonal integrity.
Antibodies targeting NFL are widely used in neuroscience research and diagnostics. They enable the detection of NFL in tissues and biofluids, serving as biomarkers for neuronal damage. Elevated NFL levels in cerebrospinal fluid (CSF) or blood correlate with neuroaxonal injury in conditions like multiple sclerosis, Alzheimer’s disease, ALS, and traumatic brain injury. These antibodies are typically monoclonal or polyclonal, validated for techniques such as immunofluorescence, Western blot, ELISA, and immunohistochemistry. Recent advances in ultrasensitive assays (e.g., SIMOA) have enhanced their utility in quantifying NFL in serum, offering a minimally invasive tool to monitor disease progression and therapeutic responses. However, interpretation requires caution, as NFL elevation is nonspecific and reflects generalized neuronal injury rather than a disease-specific signal. Ongoing research explores NFL's role in neurodegenerative mechanisms and its potential as a prognostic indicator in clinical trials.
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