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产品描述：人原癌基因酪氨酸蛋白激酶Src(SRC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SRC含量。SRC是一种原癌基因编码的非受体酪氨酸激酶。其在细胞生长、增殖、存活等过程中发挥重要作用。异常激活与肿瘤发生、发展、转移等密切相关。研究机制聚焦于其信号通路调控，开发针对SRC的抑制剂成为抗癌药物研发方向。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于基础科研领域中肿瘤发生机制探索、药物靶点筛选、细胞信号通路功能分析等研究场景，为SRC相关分子机制研究提供高效检测工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ASV ELISA Kit; Avian sarcoma virus ELISA Kit; AW259666 ELISA Kit; c SRC ELISA Kit; CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA ELISA Kit; cSrc ELISA Kit; EC 2.7.10.2 ELISA Kit; Neuronal CSRC tyrosine specific protein kinase ELISA Kit; Neuronal proto-oncogene tyrosine-protein kinase Src ELISA Kit; Neuronal SRC ELISA Kit; Oncogene SRC ELISA Kit; OTTHUMP00000174476 ELISA Kit; OTTHUMP00000174477 ELISA Kit; p60 Src ELISA Kit; p60-Src ELISA Kit; p60c-src ELISA Kit; p60Src ELISA Kit; pp60c src ELISA Kit; pp60c-src ELISA Kit; pp60csrc ELISA Kit; Proto oncogene tyrosine protein kinase Src ELISA Kit; Proto-oncogene c-Src ELISA Kit; Proto-oncogene tyrosine-protein kinase Src ELISA Kit; Protooncogene SRC ELISA Kit; Protooncogene SRC Rous sarcoma ELISA Kit; Src ELISA Kit; SRC Oncogene ELISA Kit; SRC proto oncogene non receptor tyrosine kinase ELISA Kit; SRC_HUMAN ELISA Kit; SRC1 ELISA Kit; Tyrosine kinase pp60c src ELISA Kit; Tyrosine protein kinase SRC 1 ELISA Kit; Tyrosine protein kinase SRC1 ELISA Kit; v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog ELISA Kit; V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) ELISA Kit; v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian ELISA Kit
缩写：SRC
Uniprot No.：P12931
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SRC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 86-98 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 82-93 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 82-94 | |||||
回收率：
| The recovery of human SRC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 89 | 82-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.0462.2122.1291.9458001.4961.5971.5471.3634001.0231.1021.0630.8792000.6890.6780.6840.5001000.4550.4470.4510.267500.3290.3350.3320.148250.2600.2640.2620.07800.1830.1840.184 | ||||||
货期：3-5 working days
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