Human Pancreatic secretory trypsin inhibitor(SPINK1) ELISA kit

产品详情

中文名： 人丝氨酸肽酶抑制剂，Kazal 1型(SPINK1) ELISA kit

货号: IPD-EL022575HU

Price： ￥2500

48T 96T

数量：

大包装询价

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：
人丝氨酸肽酶抑制剂，Kazal 1型(SPINK1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SPINK1含量。SPINK1是重要的研究靶点。它是一种丝氨酸蛋白酶抑制剂，在多种生理过程中发挥作用。研究发现其在胰腺癌等癌症中异常表达，可能参与肿瘤细胞的增殖、侵袭和转移，其调控机制等正成为科研重点，以探索新的治疗方向。试剂盒检测范围为78 pg/ml- 5000 pg/ml，适用于基础科研中探索SPINK1在胰腺疾病、消化道肿瘤等病理过程中的分子机制，或用于药物开发模型中评估化合物对SPINK1表达水平的调控作用，也可作为生物标志物研究的工具，为体外实验提供定量数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
ISK1_HUMAN ELISA Kit; Pancreatic secretory trypsin inhibitor ELISA Kit; Pancreatic serine protease inhibitor Kazal ELISA Kit; PCTT ELISA Kit; PSTI ELISA Kit; Serine protease inhibitor Kazal type 1 ELISA Kit; Serine protease inhibitor Kazal-type 1 ELISA Kit; Spink 3 ELISA Kit; SPINK1 ELISA Kit; Spink3 ELISA Kit; TaTI ELISA Kit; TCP ELISA Kit; Tumor associated trypsin inhibitor ELISA Kit; Tumor-associated trypsin inhibitor ELISA Kit
缩写：
SPINK1
Uniprot No.：
P00995
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/ml - 5000 pg/ml
灵敏度：
19.5 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cell Biology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SPINK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:2	Average %	95
	Range %	90-100
1:4	Average %	90
	Range %	83-97
1:8	Average %	89
	Range %	84-95
1:16	Average %	92
	Range %	88-97

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
5000	2.415	2.534	2.475	2.333
2500	1.876	1.967	1.922	1.780
1250	1.405	1.428	1.417	1.275
625	0.927	0.929	0.928	0.786
312	0.491	0.501	0.496	0.354
156	0.352	0.343	0.348	0.206
78	0.247	0.234	0.241	0.099
0	0.139	0.145	0.142