| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PRKAA2; AMPK; AMPK2; 5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK subunit alpha-2; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase |
| Entrez GeneID | 5563 |
| WB Predicted band size | Calculated MW: 62 kDa; Observed MW: 62 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human AMPK alpha 2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于AMPK α抗体的3篇代表性文献的概括(内容基于领域内常见研究主题，非真实文献)：
1. **"Characterization of AMPK α subunit-specific antibodies in knockout mouse models"**
- **作者**: Carling, D. et al.
- **摘要**: 通过AMPK α1和α2基因敲除小鼠模型，验证多种商业化AMPK α抗体的特异性。研究发现部分抗体存在交叉反应，强调需结合敲除模型验证抗体可靠性。
2. **"Tissue-specific expression and phosphorylation of AMPK α subunits revealed by validated antibodies"**
- **作者**: Steinberg, G.R. & Kemp, B.E.
- **摘要**: 利用经免疫沉淀和Western blot验证的AMPK α抗体，分析其在肌肉、肝脏等组织中的表达及磷酸化状态，揭示亚基分布差异与代谢调控的关系。
3. **"AMPK activation in energy stress: antibody-based detection and functional implications"**
- **作者**: Hawley, S.A. et al.
- **摘要**: 采用特异性AMPK α抗体研究能量应激(如葡萄糖剥夺)下AMPK的激活机制，证实α亚基Thr172位点磷酸化是活性调控关键标志。
**备注**：上述文献为示例，实际引用时请通过PubMed或学术数据库检索真实发表的论文。
AMP-activated protein kinase (AMPK) is a conserved heterotrimeric enzyme central to cellular energy homeostasis. Composed of α (catalytic), β, and γ subunits, the α-subunit exists as two isoforms (α1/PRKAA1 and α2/PRKAA2) encoded by distinct genes. The α-subunit contains an N-terminal kinase domain and a C-terminal regulatory region mediating interaction with β/γ subunits. AMPK is activated by metabolic stressors (e.g., low ATP, exercise) through phosphorylation at Thr172 in the α-subunit, triggering pathways that restore energy balance by promoting catabolism (glucose uptake, fatty acid oxidation) and inhibiting anabolism (lipogenesis, protein synthesis).
AMPK alpha antibodies are essential tools for detecting α-subunit expression, phosphorylation status, and subcellular localization. They are widely used in Western blotting, immunoprecipitation, and immunofluorescence to study AMPK activation in metabolic tissues (liver, muscle, adipose) under conditions like diabetes, obesity, and cancer. Phospho-specific antibodies targeting Thr172 are critical for assessing AMPK activity, while isoform-selective antibodies differentiate α1 (ubiquitous) from α2 (predominant in skeletal/hepatic tissues). These antibodies have advanced research into AMPK's roles in insulin signaling, mitochondrial biogenesis, and autophagy regulation, with therapeutic implications for metabolic disorders and age-related diseases. Validation using knockout controls is recommended due to potential cross-reactivity between α isoforms.
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