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产品描述：小鼠SPARC样蛋白1(SPARCL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SPARCL1含量。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于探索SPARCL1在疾病模型中的表达动态、细胞基质交互作用机制或药物干预研究，为心血管疾病、纤维化病变及肿瘤相关基础研究提供可靠工具。可满足体外实验、动物模型组织分析等科研场景需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sparcl1 ELISA Kit; Ecm2 ELISA Kit; Sc1 ELISA Kit; SPARC-like protein 1 ELISA Kit; Extracellular matrix protein 2 ELISA Kit; Matrix glycoprotein Sc1 ELISA Kit
缩写：SPARCL1
Uniprot No.：P70663
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SPARCL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-93 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-101 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 95-100 | |||||
回收率：
| The recovery of mouse SPARCL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-97 | ||||
| EDTA plasma (n=4) | 95 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.5882.5422.5652.41625001.7881.7451.7671.61812501.0791.0661.0730.9246250.6980.7010.7000.551312.50.4430.4340.4390.2901560.2980.2780.2880.139780.2220.2300.2260.07700.1500.1480.149 | ||||||
货期：3-5 working days
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