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产品描述：人SPARC(SPARC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、尿液样本中的SPARC含量。试剂盒检测范围为62.5 ng/mL-4000 ng/mL，适用于基础科研领域对SPARC功能的探索，例如肿瘤生物学研究、纤维化疾病机制分析或组织再生相关实验，研究者可通过检测不同样本中SPARC的动态变化，揭示其在疾病发生发展中的潜在作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AA517111 ELISA Kit; Basement membrane protein 40 ELISA Kit; Basement-membrane protein 40 ELISA Kit; BM 40 ELISA Kit; BM-40 ELISA Kit; BM40 ELISA Kit; Cysteine rich protein ELISA Kit; hm:zeh0062 ELISA Kit; MGC128090 ELISA Kit; OI17 ELISA Kit; ON ELISA Kit; Osteonectin ELISA Kit; Secreted acidic cystein rich glycoprotein ELISA Kit; Secreted protein acidic and cysteine rich ELISA Kit; Secreted protein acidic and rich in cysteine ELISA Kit; Secreted protein acidic cysteine rich (osteonectin) ELISA Kit; Secreted protein acidic cysteine rich ELISA Kit; SPARC ELISA Kit; SPRC ELISA Kit; SPRC_HUMAN ELISA Kit
缩写：SPARC
Uniprot No.：P09486
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, urine
检测范围：62.5 ng/mL-4000 ng/mL
灵敏度：15.6 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SPARC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 84 | |||||
| Range % | 81-89 | ||||||
| 1:2 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
| 1:4 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 95-105 | ||||||
回收率：
| The recovery of human SPARC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 87-93 | |||||
| EDTA plasma (n=4) | 101 | 97-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected40002.1202.3012.2112.04920001.6671.6421.6551.49310001.1731.1631.1681.0065000.7590.7870.7730.6112500.4530.4690.4610.2991250.3070.3010.3040.14262.50.2540.2590.2570.09500.1630.1600.162 | |||||||
货期：3-5 working days
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