| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1 |
| Entrez GeneID | 836 |
| WB Predicted band size | Calculated MW: 32 kDa; Observed MW: 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human Caspase-3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Caspase 3抗体的3篇参考文献，按文献名称、作者和摘要概括整理：
1. **"Monoclonal antibody-mediated specific detection of active Caspase-3 in apoptosis"**
- **作者**: Porter AG, Janicke RU
- **摘要**: 该文献报道了一种特异性识别活性Caspase-3的单克隆抗体的开发，验证了其在多种细胞凋亡模型(如化疗药物处理细胞)中的有效性，并证明其不与非活性酶前体结合。
2. **"Caspase-3 activation in hippocampal neurons during Alzheimer's disease pathogenesis"**
- **作者**: D’Amelio M, et al.
- **摘要**: 研究利用Caspase-3抗体通过免疫组化和Western blot技术，揭示了阿尔茨海默病小鼠模型中神经元凋亡的时空特征，表明Caspase-3活化与淀粉样斑块沉积相关。
3. **"Comparative analysis of commercial Caspase-3 antibodies for specificity and sensitivity in apoptosis assays"**
- **作者**: Smith JA, et al.
- **摘要**: 系统评估了多种市售Caspase-3抗体的性能，发现不同抗体在检测剪切形式(活化态)和全长的Caspase-3时存在显著差异，强调了抗体选择对实验可靠性的影响。
以上文献涉及抗体开发、疾病机制研究和抗体性能比较，可为Caspase-3相关实验提供参考。
Caspase 3 antibodies are essential tools for detecting and studying the activation of caspase-3. a key protease involved in programmed cell death (apoptosis). Caspase-3. a member of the cysteine-aspartic acid protease family, exists as an inactive zymogen (procaspase-3) that is cleaved into active subunits during apoptosis. Its activation marks a pivotal step in the apoptotic cascade, as it cleaves numerous cellular substrates, leading to DNA fragmentation, cytoskeletal disintegration, and eventual cell death.
Caspase 3 antibodies are designed to recognize specific epitopes of either the full-length procaspase-3 (∼35 kDa) or its cleaved active forms (∼17/19 kDa). These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to assess apoptosis in experimental models, including cancer research, neurodegenerative disease studies, and developmental biology. Specific antibodies may distinguish between inactive and active caspase-3. providing insights into the timing and extent of apoptotic signaling.
Many commercial caspase 3 antibodies are raised against synthetic peptides corresponding to conserved regions of human caspase-3. often cross-reacting with homologs in mice, rats, and other species. Validation typically includes testing in knockout models or apoptosis-induced cell lines to confirm specificity. Researchers must select antibodies based on target species, application, and the ability to detect cleaved versus full-length forms. Proper controls are critical, as nonspecific staining or cross-reactivity with other caspases (e.g., caspase-7) can occur. Overall, caspase 3 antibodies remain indispensable for elucidating apoptotic mechanisms and evaluating therapeutic interventions modulating cell death pathways.
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