首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠骨粘连蛋白(ON)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SPARC含量。SPARC 即富含半胱氨酸的酸性分泌蛋白，在多种生理和病理过程中发挥作用。其背景在于它参与细胞外基质的构建和重塑。研究机制上，SPARC 可调节细胞与基质间相互作用、影响细胞增殖与迁移，还和肿瘤的生长、转移及预后相关，是潜在研究靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于体外培养细胞分泌功能分析、骨代谢相关疾病模型研究、组织再生机制探索等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sparc ELISA Kit; SPARC ELISA Kit; Basement-membrane protein 40 ELISA Kit; BM-40 ELISA Kit; Osteonectin ELISA Kit; ON ELISA Kit; Secreted protein acidic and rich in cysteine ELISA Kit
缩写：SPARC
Uniprot No.：P07214
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ON in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 86 | ||||
| Range % | 83-89 | |||||
| 1:200 | Average % | 87 | ||||
| Range % | 82-95 | |||||
| 1:400 | Average % | 96 | ||||
| Range % | 94-98 | |||||
| 1:800 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of mouse ON spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 95-104 | ||||
| EDTA plasma (n=4) | 91 | 87-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.4772.4792.4782.342 10002.1472.1792.1632.027 5001.6941.6721.6831.547 2501.0021.0561.0290.893 1250.6330.6590.6460.510 62.50.3280.3390.3340.198 31.250.2240.2140.2190.083 00.1350.1370.136 | ||||||
货期：3-5 working days
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