首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Sortilin(SOST)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SORT1含量。试剂盒检测范围为6.25 pg/mL-400 pg/mL，该产品适用于基础科研中探究Sortilin在神经系统疾病模型、代谢紊乱动物实验或相关信号通路中的表达变化，为体外实验提供蛋白水平的定量数据支持，实验结果需结合其他分子生物学方法进一步验证。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sort1 ELISA Kit; Sortilin ELISA Kit; Neurotensin receptor 3 ELISA Kit; NTR3 ELISA Kit; mNTR3 ELISA Kit
缩写：SORT1
Uniprot No.：Q6PHU5
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SORT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 88-98 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 96-105 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-97 | |||||
回收率：
| The recovery of mouse SORT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 96 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 4002.5762.5022.5392.376 2002.1072.0062.0571.894 1001.4231.3641.3941.231 500.7290.7300.7300.567 250.4700.4340.4520.289 12.50.3260.3060.3160.153 6.250.2620.2470.2550.092 00.1570.1680.163 | ||||||
货期：3-5 working days
×
