| WB | 咨询技术 | Human,Rat,Hamster |
| IF | 咨询技术 | Human,Rat,Hamster |
| IHC | 咨询技术 | Human,Rat,Hamster |
| ICC | 技术咨询 | Human,Rat,Hamster |
| FCM | 咨询技术 | Human,Rat,Hamster |
| Elisa | 咨询技术 | Human,Rat,Hamster |
| Aliases | Serine/threonine-protein kinase GSK3A |
| Entrez GeneID | 2931 |
| WB Predicted band size | Calculated MW: 51 kDa; Observed MW: 51,47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat,Hamster |
| Immunogen | A synthetic peptide of human GSK3 alpha |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与GSK3α/β抗体相关的代表性文献，按研究主题分类简要概括：
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1. **文献名称**：*"Glycogen synthase kinase-3: properties, functions, and regulation"*
**作者**：Jope RS, Johnson GV
**摘要**：该综述详细描述了GSK3α和GSK3β的结构差异、生物学功能及其在疾病中的作用，重点讨论了针对这两种亚型的特异性抗体的应用，包括在阿尔茨海默病和糖尿病模型中检测其磷酸化状态的变化。
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2. **文献名称**：*"GSK3 takes centre stage more than 20 years after its discovery"*
**作者**：Frame S, Cohen P
**摘要**：文章系统总结了GSK3α/β在Wnt和胰岛素信号通路中的调控机制，并展示了使用特异性抗体鉴定其不同磷酸化位点(如Ser21/Ser9)的实验方法，强调了抗体在区分活性与非活性形式中的关键作用。
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3. **文献名称**：*"Tissue-specific role of glycogen synthase kinase 3β in glucose homeostasis"*
**作者**：Cross DA, Alvarez JC
**摘要**：通过构建GSK3β条件性基因敲除小鼠，研究利用GSK3α/β抗体验证组织特异性蛋白表达水平，揭示了GSK3β在肝脏和肌肉胰岛素信号传导中的差异性调控，为代谢疾病治疗提供新靶点。
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如需具体实验细节(如抗体货号或应用场景)，可进一步补充说明。
**Background of GSK3 Alpha/Beta Antibodies**
Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase with two isoforms, GSK3α and GSK3β, encoded by distinct genes. These isoforms play critical roles in cellular processes, including glycogen metabolism, Wnt/β-catenin signaling, apoptosis, and circadian rhythm regulation. GSK3α and GSK3β share 85% sequence homology but exhibit both overlapping and distinct functions. Dysregulation of GSK3 activity is linked to diseases such as Alzheimer's, diabetes, cancer, and mood disorders.
GSK3 alpha/beta antibodies are essential tools for detecting and studying these isoforms in research. They often target conserved regions (e.g., N-terminal residues) or isoform-specific sequences. A common application is detecting phosphorylation status, particularly at inhibitory sites (Ser21 for GSK3α and Ser9 for GSK3β), which reflects kinase activity. These antibodies are widely used in techniques like Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry to explore GSK3 expression, localization, and post-translational modifications across tissues or cell lines.
Many commercial GSK3 alpha/beta antibodies are validated for specificity, as cross-reactivity between isoforms or with unrelated proteins can occur. Researchers must optimize conditions (e.g., blocking buffers, sample preparation) to ensure accurate results. Additionally, isoform-selective antibodies are critical for dissecting unique roles of GSK3α and GSK3β in disease models or drug discovery. Their utility extends to studying therapeutic inhibitors, such as lithium or small-molecule compounds, which modulate GSK3 activity in pathological contexts.
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