首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Sortilin(SORT1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SORT1含量。Sortilin（SORT1）是一种在细胞内体运输中起关键作用的分拣蛋白，广泛表达于神经元、肝细胞、脂肪细胞等。其研究机制涉及脂质代谢、神经递质转运、炎症反应等多个方面，对心血管疾病、代谢性疾病等有重要影响。通过调节Sortilin的表达或功能，可能为治疗相关疾病提供新的策略。试剂盒检测范围为46.88 pg/mL-3000 pg/mL，适用于神经生物学、心血管病理机制及代谢调控相关的基础研究，例如通过分析疾病模型或药物干预后不同样本中SORT1的浓度变化，探索其分子功能及作用通路。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：100 kDa NT receptor ELISA Kit; Glycoprotein 95 ELISA Kit; Gp 95 ELISA Kit; Gp95 ELISA Kit; LDLCQ6 ELISA Kit; Neurotensin receptor 3 ELISA Kit; NT 3 ELISA Kit; NT3 ELISA Kit; NTR 3 ELISA Kit; NTR3 ELISA Kit; OTTHUMP00000013784 ELISA Kit; SORT 1 ELISA Kit; SORT_HUMAN ELISA Kit; SORT1 (gene name) ELISA Kit; Sort1 ELISA Kit; Sortilin 1 ELISA Kit; Sortilin ELISA Kit
缩写：SORT1
Uniprot No.：Q99523
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：46.88 pg/mL-3000 pg/mL
灵敏度：11.72 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human sortilin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-93 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 100-107 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 93-103 | |||||
回收率：
| The recovery of human sortilin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-97 | ||||
| EDTA plasma (n=4) | 97 | 93-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30002.3492.3332.3412.18415001.8641.7761.8201.6637501.3121.3021.3071.1503750.9430.9220.9330.776187.50.6540.6340.6440.48793.750.4630.4560.4600.30346.880.3170.3040.3110.15400.1580.1560.157 | ||||||
货期：3-5 working days
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