| WB | 咨询技术 | Human,Hamster |
| IF | 咨询技术 | Human,Hamster |
| IHC | 1/50-1/100 | Human,Hamster |
| ICC | 技术咨询 | Human,Hamster |
| FCM | 咨询技术 | Human,Hamster |
| Elisa | 咨询技术 | Human,Hamster |
| Aliases | CD8A; MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD antigen CD8a |
| Entrez GeneID | 925 |
| WB Predicted band size | Calculated MW: 26 kDa; Observed MW: 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Hamster |
| Immunogen | A synthetic peptide of human CD8 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于CD8α抗体的3篇经典文献及其摘要内容的简要概括：
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1. **文献名称**：*CD8α is essential for T cell development and differentiation*
**作者**：Fung-Leung WP, et al.
**摘要**：该研究通过基因敲除技术证明，CD8α缺失导致小鼠胸腺中CD8+ T细胞发育停滞，无法完成阳性选择，揭示了CD8α在T细胞成熟中的关键作用，并证实其与MHC I类分子互作的必要性。
2. **文献名称**：*CD8α mediates T cell signaling via interaction with Lck kinase*
**作者**：Zamoyska R, et al.
**摘要**：文章阐明了CD8α的胞内结构域通过结合Lck激酶参与T细胞活化信号传导，实验显示CD8α抗体阻断会抑制TCR下游的磷酸化级联反应，影响T细胞增殖和效应功能。
3. **文献名称**：*Targeting CD8α for cancer immunotherapy using monoclonal antibodies*
**作者**：Gattinoni L, et al.
**摘要**：该研究开发了一种抗CD8α的单克隆抗体，并在小鼠肿瘤模型中验证其增强肿瘤特异性T细胞浸润和杀伤的能力，提出CD8α抗体可作为免疫检查点调节剂用于实体瘤治疗。
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以上文献涵盖CD8α的基础生物学功能(发育、信号传导)和临床应用潜力，均为该领域的代表性研究。如需具体出版信息，建议通过PubMed或期刊数据库进一步检索。
CD8 alpha antibodies are essential tools in immunology research, specifically targeting the CD8α chain, a glycoprotein expressed on the surface of cytotoxic T lymphocytes (CTLs) and some dendritic cells. CD8α, typically forming a heterodimer with CD8β, acts as a co-receptor for MHC class I molecules, enhancing T-cell receptor (TCR) signaling during antigen recognition. This interaction is critical for CTL activation, enabling targeted killing of infected or malignant cells.
CD8 alpha antibodies are widely used to identify and isolate CD8+ T-cell subsets via flow cytometry, immunohistochemistry, or functional assays. Monoclonal antibodies against CD8α, generated using hybridoma or recombinant technologies, exhibit high specificity and are pivotal in studying T-cell development, differentiation, and effector functions. They also serve as biomarkers in disease contexts, such as viral infections, autoimmune disorders, and cancer immunotherapy. For instance, CD8+ T-cell infiltration in tumors correlates with treatment response, making these antibodies valuable in prognostic assessments.
Additionally, CD8 alpha antibodies are employed in therapeutic strategies, including bispecific antibodies or chimeric antigen receptor (CAR) T-cell designs, to redirect immune responses against specific targets. Their versatility in research, diagnostics, and emerging therapies underscores their importance in advancing immunology and precision medicine.
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