首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠超氧化物歧化酶[锰],线粒体(SOD2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SOD2含量。SOD2（超氧化物歧化酶2）是一种线粒体酶，主要功能是清除细胞内的超氧阴离子自由基，保护细胞免受氧化损伤。它在多种疾病中扮演重要角色，包括癌症。近期研究发现，SOD2在乳腺癌中可能发挥双重作用，即早期作为肿瘤抑制因子，晚期作为肿瘤促进剂，其作用机制可能与JAK-STAT信号通路相关。试剂盒检测范围为1.87 pg/mL-120 pg/mL，适用于基础研究中对氧化应激相关模型的分子机制解析、药物干预效果评估或疾病生物标志物探索等科研场景，为心血管、神经系统及代谢领域的研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sod2 ELISA Kit; Superoxide dismutase [Mn] ELISA Kit; mitochondrial ELISA Kit; EC 1.15.1.1 ELISA Kit
缩写：SOD2
Uniprot No.：P07895
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.87 pg/mL-120 pg/mL
灵敏度：0.47 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SOD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 89 | ||||
| Range % | 84-92 | |||||
| 1:2000 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:4000 | Average % | 88 | ||||
| Range % | 84-91 | |||||
| 1:8000 | Average % | 99 | ||||
| Range % | 93-102 | |||||
回收率：
| The recovery of rat SOD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-93 | ||||
| EDTA plasma (n=4) | 101 | 98-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1202.4272.3982.4132.307601.8871.8621.8751.769301.4491.4021.4261.320150.8030.8130.8080.7027.50.4920.4660.4790.3733.750.2920.2870.2900.1841.870.2020.2130.2080.10200.1070.1050.106 | ||||||
货期：3-5 working days
×
