| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ERK; p38; p40; p41; ERT1; MAPK2; PRKM1; P42MAPK; p41mapk; MAPK |
| Entrez GeneID | 5594 |
| WB Predicted band size | Calculated MW: 41 kDa; Observed MW: 41 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human ERK2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与ERK2抗体相关的文献示例(基于真实研究背景，具体作者和标题为模拟简化版)：
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1. **文献名称**：*"Selective activation of ERK2 by osmotic stress in mammalian cells"*
**作者**：Robinson MJ et al.
**摘要**：本研究通过Western blot和免疫沉淀技术，利用特异性ERK2抗体(货号：sc-154)，证明了ERK2而非ERK1在细胞渗透压应激反应中的选择性激活机制。结果显示ERK2的磷酸化水平显著上调，并参与下游基因调控。
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2. **文献名称**：*"ERK2 localization in neuronal differentiation: an immunofluorescence study"*
**作者**：Cheng X et al.
**摘要**：通过高特异性ERK2单克隆抗体(克隆号：3A7)，作者采用免疫荧光技术观察了ERK2在神经元分化过程中的亚细胞定位变化，发现其核转位与突触形成密切相关，提示ERK2在神经发育中的关键作用。
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3. **文献名称**：*"Targeting ERK2 in cancer therapy: antibody validation and functional analysis"*
**作者**：Singh A et al.
**摘要**：该研究验证了一种商业化ERK2抗体(Abcam #ab126428)在流式细胞术和免疫组化中的应用，证实其在乳腺癌组织中特异性识别ERK2蛋白，并发现ERK2高表达与患者预后不良相关。
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如需具体文献，建议通过PubMed或Google Scholar搜索关键词“ERK2 antibody validation”“ERK2 western blot”或“ERK2 immunohistochemistry”获取最新研究。
ERK2 (Extracellular Signal-Regulated Kinase 2), also known as MAPK1. is a serine/threonine kinase central to the MAPK/ERK signaling pathway, which regulates critical cellular processes including proliferation, differentiation, survival, and apoptosis. As a member of the mitogen-activated protein kinase (MAPK) family, ERK2 is activated by upstream kinases (MEK1/2) through phosphorylation at Thr185 and Tyr187. It then translocates to the nucleus to modulate transcription factors or interacts with cytoplasmic targets. ERK2 shares ~85% sequence identity with ERK1 (MAPK3) but differs in tissue distribution and substrate specificity, with ERK2 being more ubiquitously expressed and linked to cell cycle progression.
ERK2 antibodies are essential tools for studying its expression, activation, and localization in various biological contexts. These antibodies are typically developed against specific epitopes, such as the C-terminal region or phosphorylated residues, enabling detection of total ERK2 or its active (phosphorylated) form via techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). High-quality ERK2 antibodies are validated for specificity using knockout cell lines or siRNA-mediated knockdown to avoid cross-reactivity with ERK1 or other MAPKs. They are widely used in cancer research (e.g., studying hyperactivated ERK2 in tumors), neuroscience (e.g., synaptic plasticity), and drug development (e.g., MEK/ERK pathway inhibitor screening). Proper controls, such as phosphorylation status verification and sample treatment with pathway activators/inhibitors, are critical for accurate data interpretation.
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