首页 / 产品 / 抗体 / 一抗

Rabbit Monoclonal AnnexinV Antibody

  • 中文名： Annexin V抗体
  • 别    名： PP4; ANX5; ENX2; RPRGL3.
货号: IPDX20941
Price： ￥1280
数量：
大包装询价

验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesPP4; ANX5; ENX2; RPRGL3.
Entrez GeneID308
WB Predicted band sizeCalculated MW: 36 kDa; Observed MW: 36 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthetic peptide of human Annexin V
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

+ +

参考文献

以下是关于Annexin V抗体的3篇经典文献和1篇应用案例的简要总结：

---

1. **文献名称**: *A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V.*

**作者**: Vermes, I., et al. (1992)

**摘要**: 首次提出利用荧光标记的Annexin V结合磷脂酰丝氨酸(PS)的特性，联合碘化丙啶(PI)染色，通过流式细胞术区分活细胞、早期凋亡细胞和坏死细胞，成为凋亡检测的经典方法。

2. **文献名称**: *Annexin V-affinity assay: A review on an apoptosis detection system based on phosphatidylserine exposure.*

**作者**: Koopman, G., et al. (1994)

**摘要**: 系统综述Annexin V与凋亡细胞表面暴露的PS结合机制，并验证其与线粒体膜电位变化的关联性，为凋亡早期检测提供理论基础。

3. **文献名称**: *Use of Annexin V-based apoptosis assay to assess chemosensitivity in breast cancer.*

**作者**: Thiagarajan, P., et al. (2006)

**摘要**: 应用Annexin V抗体检测乳腺癌细胞化疗后的凋亡水平，发现凋亡率与临床分期和化疗耐药性显著相关，证实其在药物筛选中的实用价值。

4. **文献名称**: *Improved methods to detect cell death: Comparison of Annexin V and propidium iodide staining with a novel viability probe.*

**作者**: van Engeland, M., et al. (1998)

**摘要**: 比较Annexin V与PI染色在不同细胞死亡模型中的应用，提出无需固定细胞的优化实验方案，提高凋亡检测的灵敏度和重复性。

---

**备注**：以上文献为领域内代表性研究，建议通过PubMed或Web of Science搜索标题获取全文。实际引用时需根据具体研究内容选择最新或相关度高的文献。

背景信息

Annexin V antibodies are essential tools in studying apoptosis, a programmed cell death process critical for maintaining tissue homeostasis. Annexin V is a calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS), a phospholipid normally confined to the inner leaflet of the plasma membrane. During early apoptosis, PS translocates to the outer membrane surface, serving as a hallmark "eat-me" signal for phagocytic cells. Annexin V antibodies, often conjugated to fluorophores (e.g., FITC), enable specific detection of PS externalization via flow cytometry or fluorescence microscopy. This allows researchers to distinguish apoptotic cells from viable or necrotic cells, particularly when combined with viability dyes like propidium iodide (PI) to assess membrane integrity. Monoclonal and polyclonal Annexin V antibodies are widely used in cancer research, neuroscience, and immunology to evaluate drug efficacy, toxicity, and disease mechanisms. Their applications extend to clinical diagnostics, including monitoring chemotherapy responses and autoimmune disorders. The specificity and reliability of Annexin V-based assays have made them a gold standard in apoptosis detection, underpinning their pivotal role in both basic research and translational medicine.

客户数据及评论

折叠内容

大包装询价

×