关键信息
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基因名
ABCG5
- 应用
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别名
ABC-G5; STSL; Sterolin 1; ATP-binding cassette sub-family G member 5
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种属
Mouse
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q99PE8
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表达区间
Asp66~Gly276
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分子量
30&27kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
ABCG5 is a member of the ATP-binding cassette (ABC) transporter family, specifically involved in sterol transport, which plays a crucial role in cholesterol and phytosterol metabolism. Mutations or dysfunction in ABCG5 have been linked to diseases such as sitosterolemia, a rare genetic disorder characterized by the abnormal accumulation of plant sterols in the body, leading to premature atherosclerosis. The study of ABCG5 and its recombinant protein is significant for understanding the mechanisms of sterol absorption and the regulation of lipid homeostasis. Research on ABCG5 has focused on elucidating its structure, function, and interaction with other proteins in the cell membrane. The recombinant form of this protein allows for a detailed examination of its transport activity and the effects of specific mutations. Additionally, investigating ABCG5's role in cellular processes aids in identifying potential therapeutic targets for managing hypercholesterolemia and related metabolic disorders. As our understanding of ABCG5 deepens, it could pave the way for innovative treatments that modulate lipid metabolism and address cardiovascular diseases stemming from dyslipidemia. Thus, the research on ABCG5 recombinant protein is vital for both basic and translational science, offering insights into cholesterol regulation and possible pathways to novel therapeutic interventions.
