首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：鱼超氧化物歧化酶(SOD)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SOD含量。SOD即超氧化物歧化酶，是生物体内重要抗氧化酶。其背景在于它能清除超氧阴离子自由基，保护细胞免受氧化损伤。研究机制方面，主要是催化超氧阴离子发生歧化反应，生成氧气和过氧化氢，在抗炎症、延缓衰老等研究中受关注。试剂盒检测范围为1.25 ng/mL-20 ng/mL，该产品可为鱼类生理学、环境毒理学及水产养殖研究提供可靠工具，例如评估污染物暴露对鱼类氧化应激的影响、监测养殖水体环境压力下的生物标志物变化，或探究营养干预对鱼类抗氧化系统的调控机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：sod1 ELISA Kit; cuzn ELISA Kit; Superoxide dismutase [Cu-Zn] ELISA Kit; EC 1.15.1.1 ELISA Kit
缩写：SOD
Uniprot No.：O73872
种属：Fish
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-20 ng/mL
灵敏度：0.625 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish SOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 95 | ||||
| Range % | 92-98 | |||||
| 1:200 | Average % | 103 | ||||
| Range % | 100-106 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of fish SOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 96-103 | ||||
| EDTA plasma (n=4) | 89 | 85-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 200.0920.0990.096 100.1430.1520.148 50.2450.2230.234 2.50.5880.5750.582 1.251.3971.4021.400 02.2142.3522.283 | ||||||
货期：3-5 working days
×
