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产品描述：人细胞因子信号转导抑制因子3(SOCS-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SOCS3含量。SOCS3 是重要的细胞信号负调控因子。其参与多种生理与病理过程。研究机制上，它主要通过与细胞内相关信号通路关键分子结合，抑制 JAK - STAT 等信号通路过度激活，在炎症、肿瘤、代谢等领域是研究热点。试剂盒检测范围为47 pg/mL-3000 pg/mL，适用于体外研究SOCS - 3在炎症性疾病、代谢综合征、肿瘤微环境等领域的表达调控机制，为探索细胞因子信号网络调控、药物靶点筛选及疾病模型建立提供可靠的定量分析工具，严格限定于科学研究用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ATOD4 ELISA Kit; CIS 3 ELISA Kit; CIS-3 ELISA Kit; CIS3 ELISA Kit; Cish3 ELISA Kit; Cytokine induced SH2 protein 3 ELISA Kit; Cytokine-inducible SH2 protein 3 ELISA Kit; E2a Pbx1 target gene in fibroblasts 10 ELISA Kit; EF 10 ELISA Kit; MGC71791 ELISA Kit; SOCS 3 ELISA Kit; SOCS-3 ELISA Kit; Socs3 ELISA Kit; SOCS3_HUMAN ELISA Kit; SSI 3 ELISA Kit; SSI-3 ELISA Kit; SSI3 ELISA Kit; STAT induced STAT inhibitor 3 ELISA Kit; STAT-induced STAT inhibitor 3 ELISA Kit; Suppressor of cytokine signaling 3 ELISA Kit
缩写：SOCS3
Uniprot No.：O14543
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SOCS-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 88-99 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 82-102 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 84-105 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of human SOCS-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 83-110 | ||||
| EDTA plasma (n=4) | 97 | 86-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 30002.0692.1052.0872.002 15001.4861.5171.5021.417 7501.1081.0341.0710.986 3750.5910.6030.5970.512 187.50.3960.4080.4020.317 93.750.2860.2740.2800.195 46.880.2100.2030.2070.122 00.0830.0870.085 | ||||||
货期：3-5 working days
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