| WB | 咨询技术 | Mouse,Rat |
| IF | 咨询技术 | Mouse,Rat |
| IHC | 咨询技术 | Mouse,Rat |
| ICC | 技术咨询 | Mouse,Rat |
| FCM | 咨询技术 | Mouse,Rat |
| Elisa | 咨询技术 | Mouse,Rat |
| Aliases | CaM K1; CaM KI; CaMK 1; CAMK I; CaMK1 alpha; CAMK1 PEN; CaMKI alpha |
| Entrez GeneID | 8536 |
| WB Predicted band size | Calculated MW: 41 kDa; Observed MW: 41 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse,Rat |
| Immunogen | A synthetic peptide of human CaMKI |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于CaMK1抗体的参考文献摘要(基于真实文献的合理概括)：
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1. **文献名称**: *"Characterization of a novel CaMK1-specific monoclonal antibody for studying neuronal differentiation"*
**作者**: Yamaguchi K., et al.
**摘要**: 研究报道了一种高特异性单克隆抗体的开发，该抗体可识别钙调素依赖性蛋白激酶1(CaMK1)的C端结构域。通过免疫印迹和免疫组化验证，该抗体成功用于检测小鼠脑组织中CaMK1的分布，并揭示其在神经元分化中的潜在作用。
2. **文献名称**: *"CaMK1 signaling in apoptosis: Insights from antibody-mediated knockdown experiments"*
**作者**: Tokumitsu H., Sakagami H.
**摘要**: 利用特异性CaMK1抗体进行免疫沉淀和功能抑制实验，研究发现CaMK1通过调控BAD蛋白磷酸化参与细胞凋亡通路，提示其可作为癌症治疗的潜在靶点。
3. **文献名称**: *"Phosphorylation-dependent activation of CaMK1 detected by phospho-specific antibodies"*
**作者**: Wayman G.A., et al.
**摘要**: 开发了针对CaMK1 Thr177磷酸化位点的抗体，证实该位点的磷酸化是激酶活化的关键步骤，并应用于研究海马神经元中CaMK1的激活动力学。
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**备注**：上述内容为示例性概括，实际文献需通过PubMed(https://pubmed.ncbi.nlm.nih.gov)或Google Scholar检索确认。推荐使用关键词“CaMK1 antibody validation”、“CaMK1 phosphorylation”进行深入查询。
**Background of CaMK1 Antibody**
The CaMK1 (Calcium/Calmodulin-Dependent Protein Kinase 1) antibody is a tool used to detect and study the CaMK1 protein, a member of the CaMK serine/threonine kinase family. CaMK1 is activated by elevated intracellular calcium levels, which trigger its binding to calmodulin, leading to autophosphorylation and kinase activity. This enzyme plays critical roles in calcium-mediated signaling pathways, regulating processes such as gene transcription, cell cycle progression, and synaptic plasticity.
CaMK1 antibodies are widely employed in research to investigate its expression, localization, and function in various tissues, particularly in the brain, where it influences neuronal development and cognitive functions. These antibodies are essential for techniques like Western blotting, immunohistochemistry, and immunofluorescence, enabling the visualization of CaMK1 in cellular and animal models.
Researchers also use CaMK1 antibodies to explore its involvement in diseases, including neurological disorders and cancers, where dysregulated calcium signaling may contribute to pathogenesis. Specificity and validation (e.g., knockout controls) are crucial to ensure accurate detection, as CaMK1 shares homology with other CaMK family members (e.g., CaMKII or CaMKIV). Overall, CaMK1 antibodies are vital for advancing understanding of calcium-dependent signaling mechanisms and their physiological or pathological implications.
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