| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GAP; PKWS; RASA; CMAVM; CM-AVM; RASGAP; p120GAP; p120RASGAP |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Fusion protein of human RASA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇关于Caspase 3抗体的代表性文献及摘要概括：
1. **文献名称**：*Identification and inhibition of the ICE/CED-3 protease responsible for mammalian apoptosis*
**作者**：Nicholson DW et al.
**摘要**：该研究首次纯化并鉴定了Caspase-3(CPP32)，开发了特异性抗体用于检测其活化形式。证实Caspase-3在凋亡过程中被切割激活，并参与多聚ADP核糖聚合酶(PARP)的降解。
2. **文献名称**：*A combinatorial approach defines specificities of members of the caspase family and granzyme B*
**作者**：Thornberry NA et al.
**摘要**：通过底物特异性分析揭示了Caspase-3的酶活性特征，并利用抗体进行蛋白质定位研究。文章阐明了Caspase-3在凋亡信号中的核心作用，为抗体在酶活性检测中的应用提供依据。
3. **文献名称**：*Caspase-3 is required for DNA fragmentation and morphological changes associated with apoptosis*
**作者**：Porter AG, Jänicke RU
**摘要**：通过基因敲除和抗体免疫印迹技术，证明Caspase-3缺失导致细胞凋亡中DNA断裂和形态学改变缺陷，确立了该蛋白酶在凋亡执行阶段的必要性。
4. **文献名称**：*The apoptosome: signaling platform of cell death*
**作者**：Riedl SJ, Salvesen GS
**摘要**：综述Caspase-3在凋亡体信号通路中的激活机制，强调特异性抗体在检测其前体(procaspase-3)和活化片段中的应用，及其在疾病模型中的检测价值。
这些文献分别从功能验证、底物特异性、基因缺失模型和信号通路层面，展示了Caspase-3抗体在凋亡机制研究中的关键作用。
Caspase-3 antibody is a widely used tool in apoptosis research, targeting the effector protease caspase-3. a critical mediator of programmed cell death. Caspase-3. a member of the cysteine-aspartic acid protease (caspase) family, exists as an inactive zymogen (procaspase-3) that is cleaved into active subunits during apoptosis. Its activation triggers the cleavage of key cellular substrates, leading to morphological and biochemical hallmarks of apoptosis, such as DNA fragmentation and membrane blebbing.
Caspase-3 antibodies are designed to detect either the full-length (inactive) or cleaved (active) forms, enabling researchers to assess apoptosis in experimental models. These antibodies are essential in techniques like Western blotting, immunohistochemistry, and flow cytometry to quantify caspase-3 expression or activation in cells and tissues. They are particularly valuable in studies on cancer, neurodegenerative diseases, and developmental biology, where dysregulated apoptosis plays a central role.
Most commercial caspase-3 antibodies are raised against synthetic peptides corresponding to specific regions, such as the N-terminal prodomain or the cleavage site (e.g., Asp175 in human caspase-3). Validation often involves knockout cell lines or recombinant proteins to confirm specificity. Some antibodies distinguish between isoforms or species-specific variants, requiring careful selection based on experimental needs. Monoclonal antibodies offer high specificity, while polyclonal versions may detect broader epitopes. Proper controls, such as untreated vs. apoptosis-induced samples, are critical for interpreting results accurately.
Overall, caspase-3 antibodies remain indispensable for elucidating apoptotic mechanisms and evaluating therapeutic interventions targeting cell death pathways.
×
