| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SIRT2; SIR2L; SIR2L2; NAD-dependent protein deacetylase sirtuin-2; Regulatory protein SIR2 homolog 2; SIR2-like protein 2 |
| Entrez GeneID | 22933 |
| WB Predicted band size | Calculated MW: 43 kDa; Observed MW: 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human SIRT2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于SIRT2抗体的参考文献摘要(人工模拟示例，非真实文献)：
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1. **文献名称**：*"SIRT2 deacetylase regulates mitosis through tubulin modulation"*
**作者**：North BJ, et al.
**摘要**：本研究利用特异性SIRT2抗体(克隆号3A10)验证了SIRT2在细胞周期中的定位，发现其通过去乙酰化α-tubulin调控有丝分裂。抗体经SIRT2敲除细胞系验证特异性，免疫荧光显示SIRT2与纺锤体共定位。
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2. **文献名称**：*"SIRT2 inhibition improves metabolic syndrome in mice"*
**作者**：Verdin E, et al.
**摘要**：文章开发了一种靶向SIRT2的小分子抑制剂，并采用兔源多克隆SIRT2抗体(Abcam #1234)检测肝脏组织中SIRT2表达变化。Western blot和免疫组化显示抑制SIRT2可改善胰岛素敏感性，抗体特异性经siRNA敲低实验确认。
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3. **文献名称**：*"SIRT2 mediates axonal degeneration in Parkinson's disease models"*
**作者**：Pandithage R, et al.
**摘要**：通过小鼠单克隆SIRT2抗体(Sigma S5697)研究SIRT2在神经退行性疾病中的作用，发现其介导多巴胺能神经元轴突变性。抗体在脑组织切片中特异性标记SIRT2.并通过IP-MS验证靶标结合能力。
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如需真实文献，建议在PubMed或Google Scholar检索关键词“SIRT2 antibody validation”或“SIRT2 immunoblotting”筛选近期高引论文。
SIRT2 (sirtuin 2) is a member of the sirtuin family of NAD\(^+\)-dependent class III histone deacetylases, involved in regulating cellular processes such as genome stability, metabolism, aging, and stress responses. Primarily localized in the cytoplasm, SIRT2 deacetylates both histone (e.g., H3K18. H4K16) and non-histone substrates (e.g., α-tubulin, p300. PARP1), linking its activity to cell cycle control, mitochondrial function, and neurodegenerative disease pathways. Its role in cancer is context-dependent, acting as either an oncogene or tumor suppressor.
SIRT2 antibodies are essential tools for studying protein expression, subcellular localization, and interactions in diverse biological contexts. They are widely used in techniques like Western blotting, immunofluorescence, immunohistochemistry, and co-immunoprecipitation. Commercial SIRT2 antibodies are typically raised against specific epitopes (e.g., N-terminal, C-terminal regions) and validated for reactivity across human, mouse, or rat samples. However, specificity challenges exist due to homology among sirtuin family members, necessitating rigorous validation via knockout/knockdown controls.
Research applications include investigating SIRT2's involvement in metabolic disorders (e.g., diabetes), neurodegeneration (e.g., Parkinson’s, Alzheimer’s), and cancer progression. Recent interest also focuses on its modulation by small-molecule inhibitors/activators for therapeutic purposes. Reliable SIRT2 antibodies are critical for elucidating its dual roles in health and disease.
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