首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：人α-突触核蛋白(SNCA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SNCA含量。SNCA靶点编码α - 突触核蛋白，其基因突变或异常聚集与帕金森病等神经退行性疾病密切相关。研究机制主要围绕其在神经元中的正常功能、错误折叠与聚集过程，以及对神经细胞的毒性作用，旨在为相关疾病的防治提供方向。试剂盒检测范围为0.625 ng/mL-40 ng/mL，可广泛应用于神经退行性疾病模型构建、药物干预效果评估或蛋白表达调控机制研究等领域，为探索SNCA相关病理生理过程提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Snca ELISA Kit; Alpha-synuclein ELISA Kit
缩写：SNCA
Uniprot No.：P37377
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SNCA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 82-90 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-105 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 94-101 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of rat SNCA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-95 | ||||
| EDTA plasma (n=4) | 94 | 87-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.5322.5002.5162.403 202.0992.0122.0561.943 101.4011.4321.4171.304 50.9060.9210.9140.801 2.50.5660.5770.5720.459 1.250.3450.3580.3520.239 0.6250.2420.2370.2400.127 00.1120.1140.113 | ||||||
货期：3-5 working days
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