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Human synuclein, alpha (non A4 component of amyloid precursor) oligomer (SNCA oligomer) ELISA kit

  • 中文名： 人alpha突触核蛋白（淀粉样前体非A4成分蛋白）低聚物 (SNCA oligomer) ELISA kit
货号: IPD-E18033h
Price： ￥2500
数量：
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产品详情

  • 产品描述：人α-突触核蛋白(SNCA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的SNCA含量。SNCA 即 α-突触核蛋白，其编码基因参与突触小泡循环和多巴胺释放等。研究发现，它异常聚集形成的路易小体是帕金森病等神经退行性疾病的病理标志。其病理机制研究聚焦于蛋白错误折叠、聚集，以及对神经细胞的毒性作用方面。试剂盒检测范围为0.312 ng/mL-20 ng/mL，科研人员可将其应用于疾病模型构建、病理机制探索或药物干预效果评估等实验，例如通过检测帕金森病动物模型的体液样本，分析SNCA表达动态变化；为神经生物学、分子病理学等领域的体外研究提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：Alpha synuclein ELISA Kit; Alpha-synuclein ELISA Kit; Alpha-synuclein; isoform NACP140 ELISA Kit; alphaSYN ELISA Kit; MGC105443 ELISA Kit; MGC110988 ELISA Kit; MGC127560 ELISA Kit; MGC64356 ELISA Kit; NACP ELISA Kit; Non A beta component of AD amyloid ELISA Kit; Non A4 component of amyloid ELISA Kit; Non A4 component of amyloid precursor ELISA Kit; Non-A beta component of AD amyloid ELISA Kit; Non-A-beta component of alzheimers disease amyloid ; precursor of ELISA Kit; Non-A4 component of amyloid precursor ELISA Kit; Non-A4 component of amyloid; precursor of ELISA Kit; OTTHUMP00000218549 ELISA Kit; OTTHUMP00000218551 ELISA Kit; OTTHUMP00000218552 ELISA Kit; OTTHUMP00000218553 ELISA Kit; OTTHUMP00000218554 ELISA Kit; PARK 1 ELISA Kit; PARK 4 ELISA Kit; PARK1 ELISA Kit; PARK4 ELISA Kit; Parkinson disease (autosomal dominant; Lewy body) 4 ELISA Kit; Parkinson disease familial 1 ELISA Kit; SNCA ELISA Kit; Snca synuclein ELISA Kit; Snca synuclein; alpha (non A4 component of amyloid precursor) ELISA Kit; SYN ELISA Kit; Synuclein alpha ELISA Kit; Synuclein alpha 140 ELISA Kit; Synuclein; alpha (non A4 component of amyloid precursor) ELISA Kit; SYUA_HUMAN ELISA Kit

  • 缩写：SNCA

  • Uniprot No.：P37840

  • 种属：Homo sapiens (Human)

  • 样本类型：serum, plasma

  • 检测范围：0.312 ng/mL-20 ng/mL

  • 灵敏度：0.078 ng/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Neuroscience

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%



    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%



    Three samples of known concentration were tested in twenty assays to assess.










  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human SNCA oligomer in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.




    		SampleSerum(n=4)

    1:1Average %87

    Range %82-94


    1:2Average %92

    Range %87-99


    1:4Average %96

    Range %90-102


    1:8Average %102

    Range %96-108


  • 回收率：

    The recovery of human SNCA oligomer spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample TypeAverage % RecoveryRange

    Serum (n=5)9086-97

    EDTA plasma (n=4)9390-96

















  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/mlOD1OD2AverageCorrected  202.3682.2772.3232.161  101.8131.9051.8591.697  51.1981.1641.1811.019  2.50.7720.8010.7870.625  1.250.4510.4340.4430.281  0.6250.3280.3160.3220.160  0.3120.2410.2330.2370.075  00.1630.1610.162　  












  • 本试剂盒所含材料：


    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human SNCA antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled SNCA antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

  • 本试剂盒不含材料：



    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

    • An incubator can provide stable incubation conditions up to 37°C±5°C.

    • Centrifuge

    • Vortex

    • Squirt bottle, manifold dispenser, or automated microplate washer

    • Absorbent paper for blotting the microtiter plate


  • 货期：3-5 working days

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