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产品描述：人酸性神经鞘磷脂酶(ASM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、脑脊液（CSF）、尿液样本中的SMPD1含量。SMPD1 是一种重要靶点。它编码酸性鞘磷脂酶，与鞘脂代谢相关。研究发现其功能异常与多种疾病，如尼曼 - 匹克病等有关。其研究机制主要围绕酶活性调控、相关信号通路展开，以探寻疾病治疗新策略。试剂盒检测范围为1.25 ng/mL-80 ng/mL，可应用于基础科研领域，如尼曼 - 匹克病模型中的酶活性研究、脂质代谢通路调控分析、药物筛选对ASM表达水平的影响评估，以及脑脊液或尿液样本中ASM与神经病理学关联的探索性研究，为疾病机制解析和生物标志物开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Acid sphingomyelinase ELISA Kit; ASM ELISA Kit; ASM_HUMAN ELISA Kit; aSMase ELISA Kit; NPD ELISA Kit; Smpd1 ELISA Kit; Sphingomyelin phosphodiesterase 1 acid lysosomal ELISA Kit; Sphingomyelin phosphodiesterase ELISA Kit
缩写：SMPD1
Uniprot No.：P17405
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, cerebrospinal fluid (CSF), urine
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.31 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ASM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human ASM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.6622.5682.6152.433402.2232.3682.2962.114201.6781.6381.6581.476100.9480.9310.9400.75850.5960.5790.5880.4062.50.4430.4560.4500.2681.250.3250.3320.3290.14700.1810.1830.182 | ||||||
货期：3-5 working days
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