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Human SPARC-related modular calcium-binding protein 1(SMOC1) ELISA kit

  • 中文名： 人SPARC相关模块化钙结合蛋白1(SMOC1) ELISA kit
货号: IPD-EL021842HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人SPARC相关模块化钙结合蛋白1(SMOC1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SMOC1含量。SMOC1是一种与SPARC相关的模块化钙结合蛋白，其在小鼠的睾丸下降过程中发挥重要作用。SMOC1的表达受雄激素调节，并通过促进睾丸支持组织的生长和分化来调节睾丸的下降。研究显示，SMOC1在调节睾丸支持组织的细胞增殖和分化中扮演关键角色，是雄激素作用的重要下游分子。试剂盒检测范围为78 pg/mL-5000 pg/mL，可为发育生物学研究、肿瘤微环境调控机制探索、纤维化疾病模型构建等科研方向提供定量分析工具，特别适用于评估疾病模型中SMOC1的动态表达水平或药物干预实验的分子效应检测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Secreted modular calcium-binding protein 1 ELISA Kit; SMOC-1 ELISA Kit; SMOC1 ELISA Kit; SMOC1_HUMAN ELISA Kit; SPARC-related modular calcium-binding protein 1 ELISA Kit
  • 缩写：
    SMOC1
  • Uniprot No.：
    Q9H4F8
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SMOC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    		SampleSerum(n=4)
    1:1Average %96
    Range %92-100
    1:2Average %103
    Range %99-107
    1:4Average %90
    Range %85-98
    1:8Average %92
    Range %88-96
  • 回收率：
    The recovery of human SMOC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9590-101
    EDTA plasma (n=4)9389-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/mlOD1OD2AverageCorrected
    50002.5182.6252.5722.439
    25002.0072.1492.0781.945
    12501.3191.3841.3521.219
    6250.6750.6290.6520.519
    3120.4210.4470.4340.301
    1560.2630.2710.2670.134
    780.2020.2110.2070.074
    00.1300.1350.133

  • 货期：
    3-5 working days

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