| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | STARD1 |
| Entrez GeneID | 6770 |
| WB Predicted band size | Calculated MW: 32 kDa; Observed MW: 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human StAR |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于StAR抗体的3篇代表性文献的简要信息：
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1. **文献名称**：*Production and Characterization of a Monoclonal Antibody Against Human Steroidogenic Acute Regulatory Protein (StAR)*
**作者**：Clark BJ, et al.
**摘要**：该研究报道了针对人源StAR蛋白的单克隆抗体的开发与验证。抗体通过免疫小鼠获得，经Western blot和免疫组化验证，证实其特异性识别肾上腺和性腺组织中的StAR蛋白，可用于检测StAR在类固醇生成细胞中的定位与表达水平。
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2. **文献名称**：*Immunolocalization of StAR Protein in Rat Ovarian Cells During Follicular Development*
**作者**：Stocco DM, et al.
**摘要**：研究利用多克隆StAR抗体，通过免疫组织化学技术分析了大鼠卵巢颗粒细胞中StAR蛋白的时空表达模式。结果显示，StAR在排卵前卵泡的黄体化过程中显著上调，支持其在孕酮合成中的关键作用。
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3. **文献名称**：*Cross-Species Reactivity of Anti-StAR Antibodies: Implications for Comparative Endocrinology Studies*
**作者**：Bauer MP, et al.
**摘要**：本文评估了多种商业StAR抗体在鱼类、鸟类和哺乳动物中的交叉反应性。研究发现，部分抗体在非哺乳动物中存在非特异性结合，提示选择抗体时需结合物种特异性序列比对进行验证，以避免实验误差。
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以上文献涵盖了StAR抗体的开发、应用及跨物种研究，可为相关实验提供参考。如需具体文章，建议通过PubMed或ResearchGate平台检索标题获取全文。
StAR (Steroidogenic Acute Regulatory Protein) antibodies are essential tools in studying steroidogenesis, the process by which cholesterol is converted into steroid hormones. Discovered in the mid-1990s, StAR is a mitochondrial protein critical for transporting cholesterol across the mitochondrial membrane, a rate-limiting step in producing hormones like cortisol, aldosterone, and sex steroids. Mutations in the StAR gene cause congenital lipoid adrenal hyperplasia, a life-threatening disorder characterized by impaired steroidogenesis.
StAR antibodies are widely used in research to detect and quantify StAR protein expression in tissues such as the adrenal glands, gonads, and brain. They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence, helping to explore StAR’s regulation under physiological or pathological conditions (e.g., stress, endocrine disorders, or reproductive diseases). These antibodies also aid in studying transcriptional and post-translational mechanisms controlling StAR activity.
Commercial StAR antibodies are typically raised against specific epitopes (human, mouse, or rat) and validated for specificity and sensitivity. Challenges include cross-reactivity with related proteins or species variations, necessitating careful validation. Overall, StAR antibodies are pivotal in advancing our understanding of steroid hormone biosynthesis and related disorders.
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