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产品描述：人SPARC相关模块化钙结合蛋白2(SMOC2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SMOC2含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科学研究中SMOC2的表达水平分析，如探索其在组织再生、疾病模型或细胞功能研究中的作用机制本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：bA270C4A.1 ELISA Kit; bA37D8.1 (secreted modular calcium binding protein 2) ELISA Kit; bA37D8.1 ELISA Kit; dJ421D16.1 (novel thyroglobulin type 1 repeat containing protein) ELISA Kit; dJ421D16.1 ELISA Kit; MST117 ELISA Kit; MSTP117 ELISA Kit; MSTP140 ELISA Kit; Secreted modular calcium binding protein 2 ELISA Kit; Secreted modular calcium-binding protein 2 ELISA Kit; SMAP 2 ELISA Kit; SMAP-2 ELISA Kit; SMAP2 ELISA Kit; SMOC 2 ELISA Kit; SMOC-2 ELISA Kit; SMOC2 ELISA Kit; SMOC2_HUMAN ELISA Kit; Smooth muscle associated protein 2 ELISA Kit; Smooth muscle-associated protein 2 ELISA Kit; SPARC related modular calcium binding 2 ELISA Kit; SPARC-related modular calcium-binding protein 2 ELISA Kit
缩写：SMOC2
Uniprot No.：Q9H3U7
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SMOC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 96-104 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-97 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of human SMOC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-95 | ||||
| EDTA plasma (n=4) | 94 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.2722.2112.2422.09451.7941.7651.7801.6322.51.1901.1231.1571.0091.250.7870.7540.7710.6230.6250.4930.4820.4880.3400.3120.3360.3480.3420.1940.1560.2360.2210.2290.08100.1490.1470.148 | ||||||
货期：3-5 working days
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