首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Slit2酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SLIT2含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品适用于基础科研中Slit2相关信号通路研究，如神经再生模型、肿瘤微环境分析或心血管疾病机制探索，尤其适用于通过血清/血浆样本评估体内Slit2水平动态变化，或通过组织匀浆分析局部表达特征。为神经生物学、肿瘤学及发育生物学研究提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Slit2 ELISA Kit; Slit homolog 2 protein ELISA Kit; Slit-2) [Cleaved into: Slit homolog 2 protein N-product; Slit homolog 2 protein C-product] ELISA Kit
缩写：SLIT2
Uniprot No.：Q9R1B9
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Slit2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 97-104 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-99 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of mouse Slit2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-96 | ||||
| EDTA plasma (n=4) | 96 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.2862.2572.2722.120 101.8081.8221.8151.663 51.2041.1381.1711.019 2.50.8010.7870.7940.642 1.250.5070.4950.5010.349 0.6250.3500.3670.3590.207 0.3120.2500.2610.2560.104 00.1530.1510.152 | ||||||
货期：3-5 working days
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