首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Slit2酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SLIT2含量。试剂盒检测范围为0.78 ng/mL-50 ng/mL，本试剂盒适用于神经系统发育、肿瘤血管生成机制、创伤修复等基础研究领域，为研究Slit2在神经生物学、肿瘤生物学及再生医学中的功能提供了可靠的检测工具，特别适用于体外实验模型中Slit2蛋白表达变化的动态监测及不同样本间的定量比较分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Drad 1 ELISA Kit; E030015M03Rik ELISA Kit; E130320P19Rik ELISA Kit; FLJ14420 ELISA Kit; OTTHUMP00000158695 ELISA Kit; OTTHUMP00000217852 ELISA Kit; OTTHUMP00000217853 ELISA Kit; OTTHUMP00000217854 ELISA Kit; Slil 3 ELISA Kit; Slil3 ELISA Kit; Slit 2 ELISA Kit; Slit homolog 2 (Drosophila) ELISA Kit; Slit homolog 2 ELISA Kit; Slit homolog 2 protein ELISA Kit; Slit homolog 2 protein C-product ELISA Kit; Slit-2 ELISA Kit; Slit2 ELISA Kit; SLIT2_HUMAN ELISA Kit
缩写：SLIT2
Uniprot No.：O94813
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Slit2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-95 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-103 | |||||
回收率：
| The recovery of human Slit2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-96 | ||||
| EDTA plasma (n=4) | 95 | 91-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.9572.9122.9352.770252.3132.2842.2992.13412.51.4721.3881.4301.2656.250.7970.8040.8010.6363.120.4000.4060.4030.2381.560.2850.3020.2940.1290.780.2540.2440.2490.08400.1670.1630.165 | ||||||
货期：3-5 working days
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