首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人小而密低密度脂蛋白（sLDL）酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、唾液、尿液、组织匀浆样本中的sLDL含量。sLDL（小而密低密度脂蛋白）是一种特殊的脂蛋白，其颗粒较小、密度较高，易被氧化且更容易进入血管壁，在动脉粥样硬化等心血管疾病的发生发展中起重要作用。目前研究多聚焦于其致动脉粥样硬化的机制以及降低 sLDL 水平的干预措施。试剂盒检测范围为0.312 nmol/mL-20 nmol/mL，支持科研人员高效开展心血管疾病发病机制研究、降脂药物作用效果评估、代谢综合征相关模型构建等实验。适用于体外实验环境下的基础研究、药理学研究及生物标志物探索等科研场景。为纵向追踪研究或大规模样本分析提供便利。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：sLDL
种属：Homo sapiens (Human)
样本类型：serum, plasma, saliva, urine, tissue homogenates
检测范围：0.312 nmol/mL-20 nmol/mL
灵敏度：0.078 nmol/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human sLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 95 | ||||
| Range % | 92-103 | |||||
| 1:200 | Average % | 92 | ||||
| Range % | 86-96 | |||||
| 1:400 | Average % | 104 | ||||
| Range % | 97-107 | |||||
| 1:800 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of human sLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 83-93 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| nmol/mlOD1OD2Average 200.0860.0850.086 100.1770.1810.179 50.3840.3750.380 2.50.7180.7040.711 1.251.2531.2691.261 0.6251.7281.7141.721 0.3122.2232.1212.172 02.5262.4252.476 | ||||||
货期：3-5 working days
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