首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人组蛋白RNA发夹结合蛋白(SLBP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SLBP含量。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础研究领域中细胞周期调控机制探索、组蛋白代谢相关疾病模型构建（如肿瘤发生机制研究）、干细胞分化调控分析等方向，通过精准检测样本中SLBP表达水平的变化，为相关分子机制研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hairpin binding protein histone ELISA Kit; HBP ELISA Kit; Histone binding protein ELISA Kit; Histone RNA hairpin binding protein ELISA Kit; Histone RNA hairpin-binding protein ELISA Kit; Histone stem-loop-binding protein ELISA Kit; SLBP ELISA Kit; SLBP_HUMAN ELISA Kit; Stem loop (histone) binding protein ELISA Kit; Stem loop binding protein ELISA Kit
缩写：SLBP
Uniprot No.：Q14493
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SLBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human SLBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.3382.3982.3682.2065001.5871.5991.5931.4312500.9490.9680.9590.7971250.5990.5890.5940.43262.50.4250.4360.4310.26931.250.2930.3160.3050.14315.60.2310.2220.2270.06500.1610.1630.162 | ||||||
货期：3-5 working days
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