首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人唾液酸结合Ig样凝集素8(SIGLEC8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SIGLEC8含量。SIGLEC8是一个重要靶点。它主要表达于嗜酸性粒细胞等免疫细胞表面，在调节免疫反应中发挥作用。研究表明，针对SIGLEC8靶向治疗有望用于过敏、哮喘等疾病，通过阻断相关信号通路来抑制免疫细胞活性，从而改善疾病症状。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于基础研究中探索SIGLEC8在呼吸道疾病、自身免疫疾病中的分子机制，或用于体外药物筛选模型中评估靶向治疗药物对SIGLEC8通路的影响，为免疫调节研究及生物标志物分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD329 antigen ELISA Kit; CDw329 ELISA Kit; SAF 2 ELISA Kit; SAF-2 ELISA Kit; SAF2 ELISA Kit; Sialic acid binding Ig like lectin 8 ELISA Kit; Sialic acid-binding Ig-like lectin 8 ELISA Kit; Sialoadhesin family member 2 ELISA Kit; SIGL8_HUMAN ELISA Kit; Siglec 8 ELISA Kit; Siglec-8 ELISA Kit; SIGLEC8 ELISA Kit; SIGLEC8L ELISA Kit
缩写：SIGLEC8
Uniprot No.：Q9NYZ4
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SIGLEC8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 100 | |||||
| Range % | 95-107 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:4 | Average % | 84 | |||||
| Range % | 80-92 | ||||||
| 1:8 | Average % | 88 | |||||
| Range % | 84-92 | ||||||
回收率：
| The recovery of human SIGLEC8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 90-100 | |||||
| EDTA plasma (n=4) | 90 | 85-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.1642.2462.2052.04051.6181.6341.6261.4612.51.2011.1751.1881.0231.250.7920.8230.8080.6430.6250.5580.5710.5650.4000.3120.4190.4420.4310.2660.1560.3240.3050.3150.15000.1620.1670.165 | |||||||
货期：3-5 working days
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