首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠性激素结合球蛋白(SHBG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的SHBG含量。SHBG（性激素结合球蛋白），能特异性结合性激素，调节其生物活性和代谢。在研究机制方面，它与体内性激素水平相关，其表达受多种激素调节。SHBG水平变化在代谢性疾病、生殖系统疾病等研究中受关注，为相关疾病诊治提供潜在靶点。试剂盒检测范围为375 ng/mL-6000 ng/mL，适用于科研领域研究SHBG在性激素代谢调控中的作用；可用于探究性腺疾病模型、激素代谢异常机制、药物干预对性激素分布的影响等研究方向，为生殖生理学、内分泌学及药理学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Shbg ELISA Kit; Sex hormone-binding globulin ELISA Kit; SHBG ELISA Kit; Sex steroid-binding protein ELISA Kit; SBP ELISA Kit; Testis-specific androgen-binding protein ELISA Kit; ABP ELISA Kit
缩写：SHBG
Uniprot No.：P08689
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：375 ng/mL-6000 ng/mL
灵敏度：375 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 85-90 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:8 | Average % | 83 | ||||
| Range % | 80-86 | |||||
回收率：
| The recovery of rat SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 82-91 | ||||
| EDTA plasma (n=4) | 95 | 89-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 60000.2040.2010.203 30000.3360.3410.339 15000.7890.7720.781 7501.8001.8061.803 3752.3122.3532.333 | ||||||
货期：3-5 working days
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