| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Sorting nexin-5 |
| Entrez GeneID | 27131 |
| WB Predicted band size | Calculated MW: 47 kDa; Observed MW: 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human SNX5 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于SNX5抗体的3篇参考文献的简要概括：
1. **"Sorting Nexin 5 regulates amyloid precursor protein trafficking and processing in Alzheimer's disease models"**
- **作者**: Wang et al. (2020)
- **摘要**: 该研究利用SNX5抗体进行免疫共沉淀和免疫荧光实验，发现SNX5通过调控APP(β-淀粉样前体蛋白)的胞内运输，影响β-淀粉样蛋白的生成，提示SNX5在阿尔茨海默病病理中的潜在作用。
2. **"SNX5 mediates PDGFRα lysosomal sorting and promotes cancer cell proliferation"**
- **作者**: Chen & Liu (2018)
- **摘要**: 通过Western blot和免疫染色实验，作者发现SNX5抗体检测到SNX5与PDGFRα(血小板衍生生长因子受体α)的相互作用，揭示SNX5通过调控受体酪氨酸激酶的溶酶体降解途径影响肿瘤细胞增殖。
3. **"Structural basis of SNX5-PX domain interaction with phosphoinositides"**
- **作者**: Park et al. (2019)
- **摘要**: 该研究通过抗体标记的蛋白质纯化及结构分析，解析了SNX5的PX结构域与磷脂酰肌醇的结合机制，阐明了其在膜运输中的分子基础。
4. **"SNX5 deficiency enhances antiviral innate immunity by modulating TBK1 activation"**
- **作者**: Zhang et al. (2021)
- **摘要**: 使用SNX5抗体进行基因敲除验证实验，研究发现SNX5通过负调控TBK1信号通路抑制抗病毒免疫反应，为病毒感染治疗提供新靶点。
(注：以上文献信息为模拟生成，实际引用需核对真实文献。)
SNX5 (Sorting Nexin 5) is a member of the sorting nexin family, which comprises proteins involved in membrane trafficking, endosomal sorting, and cellular signaling. Characterized by a conserved phox homology (PX) domain, SNX5 facilitates interactions with phosphatidylinositol lipids on membranes, guiding its role in vesicle formation and cargo transport. It is particularly associated with the retromer complex, working alongside proteins like SNX1/SNX6 to regulate the retrieval of cargo receptors from endosomes to the trans-Golgi network. SNX5 also interacts with receptors such as EGFR and β2-adrenergic receptors, influencing their trafficking and signaling dynamics.
Antibodies targeting SNX5 are essential tools for studying its expression, localization, and function. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate SNX5's involvement in cellular processes, including endocytosis, autophagy, and receptor recycling. Research has linked SNX5 dysregulation to diseases such as cancer, where altered expression may affect cell proliferation and metastasis, and neurodegenerative disorders, potentially due to impaired protein sorting. Commercial SNX5 antibodies are often validated for specificity across human, mouse, and rat models, enabling cross-species studies. Recent work also explores SNX5's role in organelle dynamics and its interplay with other trafficking regulators, highlighting its broader impact on cellular homeostasis.
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