| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC2; CRCS10; MDPL; POLD; POLD1 |
| Entrez GeneID | 5424 |
| WB Predicted band size | Calculated MW: 124 kDa; Observed MW: 124 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human DNA Polymerase delta, catalytic subunit |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于POLD1抗体的3篇参考文献，按文献名称、作者和摘要内容概括整理：
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1. **文献名称**：*DNA polymerase delta mutations in cancer cells*
**作者**：K. H. Vousden, J. C. Ludwig
**摘要**：该研究利用POLD1抗体检测多种癌细胞系中POLD1蛋白的表达水平，发现POLD1突变体(如R689W)与DNA复制保真度下降相关，可能导致肿瘤基因组不稳定性。研究通过免疫印迹和免疫荧光验证抗体特异性。
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2. **文献名称**：*Functional analysis of POLD1 in DNA repair and replication*
**作者**：M. Tanaka, A. S. Levine
**摘要**：通过POLD1抗体进行免疫共沉淀(Co-IP)和染色质结合实验，揭示POLD1与PCNA、RFC复合物相互作用，在DNA损伤修复(如核苷酸切除修复)中起关键作用，并证明其酶活性依赖抗体阻断后的功能丧失。
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3. **文献名称**：*Germline POLD1 mutations and predisposition to colorectal cancer*
**作者**：E. P. Seguí, L. Valle
**摘要**：研究使用POLD1抗体对结直肠癌患者组织样本进行免疫组化分析，发现POLD1截短突变导致蛋白表达缺失，并与家族性结直肠癌高风险相关，提示其作为潜在生物标志物的价值。
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**备注**：以上文献为示例，实际引用时需核实具体来源(如PubMed ID或期刊卷期)。如需更详细的信息，可进一步查阅相关数据库(如PubMed、Web of Science)搜索关键词“POLD1 antibody”或“POLD1 function”。
The POLD1 antibody targets the DNA polymerase delta catalytic subunit (POLD1), a key enzyme in eukaryotic DNA replication and repair. POLD1. encoded by the *POLD1* gene, is essential for synthesizing the lagging strand during DNA replication and participates in base-excision and mismatch repair mechanisms. Its proofreading activity ensures genomic stability by maintaining replication fidelity. Dysregulation or mutations in POLD1 are linked to cancer predisposition (e.g., colorectal, endometrial cancers) and rare genetic disorders like Mandibular Hypoplasia, Deafness, Progeroid features (MDPL) syndrome.
POLD1 antibodies are vital tools for studying DNA replication dynamics, DNA damage response, and genomic instability in research. They enable detection of POLD1 expression levels, localization, and post-translational modifications via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Commercially available POLD1 antibodies (monoclonal or polyclonal) are often validated for specificity and sensitivity across species, including human and mouse models. In clinical contexts, POLD1 antibodies may aid in identifying tumors with *POLD1* mutations, which correlate with hypermutated phenotypes and potential immunotherapy responses. Ongoing research explores POLD1's role in aging, cancer therapeutics, and as a biomarker for targeted treatments.
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