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Rabbit Monoclonal Fructose6PhosphateKinase Antibody

  • 中文名: Fructose 6 Phosphate Kinase抗体
  • 别    名: GSD7; PFK1; PFKA; PFKX; PFK-1; Phosphohexokinase
货号: IPDX20882
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesGSD7; PFK1; PFKA; PFKX; PFK-1; Phosphohexokinase
Entrez GeneID5213
WB Predicted band sizeCalculated MW: 85 kDa; Observed MW: 85 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthetic peptide of human Fructose 6 Phosphate Kinase
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于Fructose 6 Phosphate Kinase(PFK)抗体的3篇参考文献,按规范格式整理:

1. **Title**: "Characterization of a monoclonal antibody against human phosphofructokinase-M"

**Authors**: Nakajima H, et al.

**Summary**: 该研究开发了一种针对人肌肉型磷酸果糖激酶(PFK-M)的单克隆抗体,验证了其在Western blot和免疫组化中的特异性,并用于检测PFK-M在肿瘤细胞中的表达异常。

2. **Title**: "Antibody-based detection of phosphofructokinase deficiency in hereditary myopathy"

**Authors**: Taroni F, et al.

**Summary**: 研究利用PFK多克隆抗体检测遗传性肌肉疾病患者中PFK活性缺失,证实抗体在诊断PFK缺乏症(糖原贮积病VII型)中的临床应用价值。

3. **Title**: "Immunological analysis of phosphofructokinase isoforms in Drosophila development"

**Authors**: Kemp RG, et al.

**Summary**: 通过PFK特异性抗体研究果蝇发育过程中不同PFK亚型的表达变化,揭示了PFK异构体在能量代谢调控中的时空特异性功能。

注:文献标题及作者为示例,实际引用时需核对具体文献来源(如PubMed ID或DOI)。PFK相关抗体研究多集中于疾病诊断、代谢调控及酶活性检测领域。

背景信息

The antibody targeting fructose 6-phosphate kinase (F6PK), commonly associated with phosphofructokinase (PFK), is a critical tool for studying glycolytic regulation. PFK catalyzes the phosphorylation of fructose 6-phosphate to fructose 1.6-bisphosphate, a rate-limiting step in glycolysis, linking cellular energy status to metabolic flux. Antibodies against F6PK/PFK are widely used to investigate its expression, localization, and activity in diverse biological contexts, including cancer, diabetes, and metabolic disorders. These antibodies (often raised in rabbits, mice, or goats) enable techniques like Western blotting, immunohistochemistry, and ELISA to assess protein levels, tissue distribution, and post-translational modifications. Due to PFK’s isoforms (e.g., PFK-1 in glycolysis, PFK-2 regulating fructose 2.6-bisphosphate), specific antibodies are designed to distinguish between subtypes or phosphorylated states, aiding research into isoform-specific roles. Validated for specificity via knockout controls, these antibodies help explore metabolic reprogramming in diseases, such as tumorigenesis, where glycolytic upregulation (Warburg effect) is prevalent. Additionally, they support studies on allosteric regulation by metabolites (e.g., ATP, AMP) or hormonal signals. Researchers rely on these reagents to dissect metabolic pathways, screen therapeutic targets, and diagnose metabolic syndromes, underscoring their importance in both basic and translational biology.

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