首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子性激素结合球蛋白(SHBG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SHBG含量。SHBG即性激素结合球蛋白，其可与雄激素、雌激素等结合并调节其活性。在它影响体内性激素的分布及代谢。研究机制上，涉及探讨其基因表达调控、与性激素结合的分子机制，以及在生殖、代谢等疾病中的作用机制。试剂盒检测范围为25 pmol/mL-400 pmol/mL，适用于评估实验动物性激素代谢状态、研究生殖系统疾病模型或药物干预对激素运输通路的影响，为生殖生理机制、内分泌调控及相关代谢疾病的基础研究提供高效分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SHBG ELISA Kit; Sex hormone-binding globulin ELISA Kit; SHBG ELISA Kit; Sex steroid-binding protein ELISA Kit; SBP ELISA Kit; Testis-specific androgen-binding protein ELISA Kit; ABP ELISA Kit
缩写：SHBG
Uniprot No.：P15196
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：25 pmol/mL-400 pmol/mL
灵敏度：25 pmol/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 90-103 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 83-99 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 90-101 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 85-100 | ||||||
回收率：
| The recovery of rabbit SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 87-100 | |||||
| EDTA plasma (n=4) | 95 | 89-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pmol/mlOD1OD2Average 4000.2660.2520.259 2000.5280.5410.535 1000.8740.8630.869 501.3481.3251.337 252.5112.5522.532 | |||||||
货期：3-5 working days
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