首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔肺表面活性物质相关蛋白A(SFTPA1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SFTPA1含量。SFTPA1，即肺表面活性物质相关蛋白A，是一种在肺泡II型上皮细胞中合成的蛋白质，主要功能是降低肺泡表面张力，维持肺泡稳定性。其研究涉及肺泡生理、病理及疾病治疗等领域。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于科研领域中对兔呼吸道疾病模型的机制研究，如评估肺损伤修复过程中 SFTPA1 的动态变化，探究感染或炎症状态下肺泡蛋白的调控规律，或用于药物干预实验中生物标志物的定量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SFTPA1 ELISA Kit; SFTP1 ELISA Kit; SFTPA ELISA Kit; Pulmonary surfactant-associated protein A ELISA Kit; PSAP ELISA Kit; PSP-A ELISA Kit; SP-A ELISA Kit
缩写：SFTPA1
Uniprot No.：P12842
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SFTPA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 82-96 | |||||
| 1:2 | Average % | 107 | ||||
| Range % | 90-111 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 85-95 | |||||
回收率：
| The recovery of rabbit SFTPA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 82-95 | ||||
| EDTA plasma (n=4) | 99 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.1101.9912.0511.950501.8131.6881.7511.650251.3821.4841.4331.33212.51.0531.0281.0410.9406.250.7260.7050.7160.6153.120.5360.5280.5320.4311.560.3090.3020.3060.20500.0980.1040.101 | ||||||
货期：3-5 working days
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