| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Centromere autoantigen A; Centromere protein A; CENP-A |
| Entrez GeneID | 1058 |
| WB Predicted band size | Calculated MW: 16 kDa; Observed MW: 16 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human CENPA |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于CENPA抗体的3-4篇文献的简要总结：
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1. **文献名称**：*CENP-A is essential for centromere function in human cells*
**作者**：Foltz, D.R. 等 (2006)
**摘要**：研究利用CENPA抗体进行染色质免疫沉淀(ChIP)和免疫荧光分析，揭示CENPA在人类细胞着丝粒组装中的关键作用，并证明其缺失导致染色体分离异常。
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2. **文献名称**：*Structural determinants for generating centromeric chromatin*
**作者**：Black, B.E. 等 (2004)
**摘要**：通过CENPA抗体结合X射线晶体学，解析CENPA组蛋白变体的结构特征，阐明其通过特异性识别着丝粒区域DNA维持基因组稳定性的机制。
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3. **文献名称**：*CENP-A overexpression promotes aneuploidy and cancer metastasis*
**作者**：Fukagawa, T. 和 Straight, A.F. 等 (2015)
**摘要**：利用CENPA抗体在多种癌症模型中验证其过表达与染色体不稳定性的关联，发现CENPA异常表达可能通过驱动非整倍体促进肿瘤转移。
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4. **文献名称**：*Epigenetic regulation by centromeric histone variants*
**作者**：Mendiratta, S. 等 (2021)
**摘要**：综述CENPA抗体在表观遗传学研究中的应用，强调其在解析着丝粒区域染色质状态及细胞分裂调控中的工具价值。
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以上文献涵盖CENPA的功能、结构、疾病关联及技术应用，均为该领域的重要参考。
CENPA (Centromere Protein A) is a histone H3 variant that replaces canonical H3 in centromeric nucleosomes, playing a critical role in specifying centromere identity and ensuring accurate chromosome segregation during cell division. As a key epigenetic marker, CENPA is essential for recruiting other centromere-associated proteins and forming the kinetochore complex, which mediates attachment to spindle microtubules. Dysregulation of CENPA is linked to chromosomal instability, aneuploidy, and cancer progression.
CENPA antibodies are vital tools for studying centromere biology, mitotic mechanisms, and diseases associated with chromosome missegregation. They are widely used in techniques like immunofluorescence, chromatin immunoprecipitation (ChIP), and Western blotting to detect CENPA localization, expression levels, and interactions. In research, these antibodies help elucidate how CENPA maintains centromere integrity, regulates cell cycle checkpoints, and contributes to epigenetic inheritance. Clinically, CENPA overexpression has been observed in various cancers, making its antibodies potential diagnostic or prognostic markers.
Both monoclonal and polyclonal CENPA antibodies are available, often validated for species specificity (human, mouse, etc.). Researchers prioritize antibodies with high specificity due to CENPA's structural similarity to histone H3. Proper controls, such as siRNA knockdown or knockout cell lines, are recommended to confirm signal authenticity. Commercial CENPA antibodies are commonly used in studies exploring cancer biology, developmental defects, and therapeutic targeting of centromere dysfunction.
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