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产品描述：人丝氨酸/精氨酸富集剪接因子1(SFRS1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SFRS1含量。SFRS1（丝氨酸/精氨酸富集剪接因子1）是一种调控RNA剪接的关键因子，与多种癌症的发生发展密切相关。其通过调控VEGFA mRNA的可变剪接，促进食管鳞状细胞癌细胞的增殖、侵袭和迁移。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于基础科研中SFRS1功能研究，如肿瘤微环境调控、神经退行性疾病模型或细胞应激反应中的剪接机制探索，为分子生物学、病理学及药物开发等领域的蛋白表达分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alternative splicing factor 1 ELISA Kit; Alternative-splicing factor 1 ELISA Kit; arginine/serine-rich 1 ELISA Kit; ASF 1 ELISA Kit; ASF ELISA Kit; ASF-1 ELISA Kit; ASF1 ELISA Kit; FLJ53078 ELISA Kit; MGC5228 ELISA Kit; P33 subunit ELISA Kit; Pre mRNA splicing factor SF2 P33 subunit ELISA Kit; pre-mRNA-splicing factor SF2 ELISA Kit; Serine/arginine-rich splicing factor 1 ELISA Kit; SF2 ELISA Kit; SF2P33 ELISA Kit; SFRS1 ELISA Kit; Splicing factor 2 alternate splicing factor ELISA Kit; Splicing factor 2 ELISA Kit; Splicing factor ELISA Kit; Splicing factor arginine/serine rich 1 ELISA Kit; SR Splicing factor 1 ELISA Kit; SRp30a ELISA Kit; srsf1 ELISA Kit; SRSF1_HUMAN ELISA Kit
缩写：SFRS1
Uniprot No.：Q07955
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SFRS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 88-97 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 90-101 | ||||||
| 1:4 | Average % | 99 | |||||
| Range % | 96-104 | ||||||
| 1:8 | Average % | 102 | |||||
| Range % | 96-106 | ||||||
回收率：
| The recovery of human SFRS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 89-98 | |||||
| EDTA plasma (n=4) | 98 | 95-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20001.8751.9691.9221.76610001.1961.2321.2141.0585000.8230.8770.8500.6942500.6350.6250.6300.4741250.3940.3850.3900.23462.50.2870.2910.2890.13331.250.2150.2220.2190.06300.1610.1510.156 | |||||||
货期：3-5 working days
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