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Rabbit Monoclonal WTAP Antibody

  • 中文名: WTAP抗体
  • 别    名: hFL(2)D; WT1-associated protein; Wilms tumor 1-associating protein; Pre-mRNA-splicing regulator WTAP
货号: IPDX20858
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliaseshFL(2)D; WT1-associated protein; Wilms tumor 1-associating protein; Pre-mRNA-splicing regulator WTAP
Entrez GeneID9589
WB Predicted band sizeCalculated MW: 44 kDa; Observed MW: 55 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenRecombinant protein of human WTAP
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是3篇与WTAP抗体相关的文献及其摘要内容的简要概括:

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1. **文献名称**:*WTAP is a novel oncogenic protein in acute myeloid leukemia*

**作者**:Li Y, et al.

**摘要**:该研究通过Western blot和免疫组化分析,使用WTAP特异性抗体揭示了WTAP在急性髓系白血病(AML)中的高表达,并证实其通过调控m6A修饰促进白血病细胞增殖和凋亡抵抗的机制。

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2. **文献名称**:*WTAP facilitates ovarian cancer progression via m6A-dependent post-transcriptional modification of EIF3C*

**作者**:Chen T, et al.

**摘要**:研究利用WTAP抗体进行免疫沉淀和染色质共沉淀实验,发现WTAP通过介导EIF3C的m6A修饰促进卵巢癌转移,为靶向WTAP的抗体在癌症治疗中的应用提供了实验依据。

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3. **文献名称**:*Structural basis for recognition of the human m6A methylation complex by WTAP antibodies*

**作者**:Wang X, et al.

**摘要**:该文献报道了一种高亲和力的WTAP单克隆抗体的开发,通过冷冻电镜和免疫荧光技术解析了抗体与WTAP蛋白复合物的结合表位,为研究WTAP的分子功能提供了工具。

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这些文献涵盖了WTAP抗体在疾病机制研究、靶向治疗探索及结构生物学中的应用。如需具体文献DOI或补充更多条目,可进一步说明。

背景信息

The WTAP (Wilms' tumor 1-associated protein) antibody is a crucial tool for studying the functional roles of the WTAP protein, a conserved nuclear factor involved in diverse cellular processes. WTAP interacts with Wilms' tumor 1 (WT1) protein, a transcription factor linked to kidney development and cancer, and serves as a core component of the RNA N6-adenosine methyltransferase (m6A) complex. This complex regulates post-transcriptional RNA modification, influencing mRNA splicing, stability, and translation. WTAP also participates in cell cycle regulation, embryonic development, and X chromosome inactivation. Dysregulation of WTAP is associated with cancers (e.g., leukemia, glioblastoma, and solid tumors) and developmental disorders. Researchers use WTAP antibodies in techniques like Western blot, immunohistochemistry (IHC), and immunoprecipitation (IP) to detect WTAP expression, localization, and interaction partners. These studies help elucidate its role in disease mechanisms and potential therapeutic targeting. Commercial WTAP antibodies are typically validated for specificity against human, mouse, or rat samples, with applications spanning cancer biology, epigenetics, and RNA metabolism research.

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