首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人肝素辅因子Ⅱ(HCⅡ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SERPIND1含量。SERPIND1 是重要靶点。它属于丝氨酸蛋白酶抑制剂家族，参与凝血、纤溶等生理过程的调控。研究聚焦其与疾病关联机制，如在血栓性疾病等发生发展中的作用，探索通过干预它来调节相关生理过程，为治疗相关疾病提供新思路。试剂盒检测范围为1.25 pg/mL-80 pg/mL，本试剂盒适用于基础医学研究，可兼容多种样本类型，包括EDTA或肝素抗凝处理的血浆样本，以及经离心预处理的动物或人体组织匀浆，为探索HCⅡ在心血管疾病、凝血异常或肝脏代谢相关研究提供可靠工具。实验操作基于标准ELISA流程，通过抗原 - 捕获抗体 - 检测抗体的特异性结合，结合酶标仪读取吸光度值实现定量分析，实验结果稳定且重复性良好，可满足体外实验中对HCⅡ表达水平动态监测的需求，例如药物干预、基因编辑或病理模型中的分子机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：D22S673 ELISA Kit; HC II ELISA Kit; HC-II ELISA Kit; HC2 ELISA Kit; HCF2 ELISA Kit; HEP2_HUMAN ELISA Kit; Heparin cofactor 2 ELISA Kit; Heparin cofactor II ELISA Kit; HLS2 ELISA Kit; Leuserpin 2 ELISA Kit; LS2 ELISA Kit; Protease inhibitor leuserpin 2 ELISA Kit; Protease inhibitor leuserpin-2 ELISA Kit; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1 ELISA Kit; Serpin D1 ELISA Kit; serpin peptidase inhibitor, clade D (heparin cofactor), member 1 ELISA Kit; SERPIND1 ELISA Kit; THPH10 ELISA Kit
缩写：SERPIND1
Uniprot No.：P05546
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 pg/mL-80 pg/mL
灵敏度：0.312 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HCⅡ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 92 | ||||
| Range % | 86-98 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 90-105 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 89-100 | |||||
回收率：
| The recovery of human HCⅡspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-102 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected802.7182.7852.7522.668402.2392.1432.1912.107201.3611.3871.3741.290100.7580.7420.7500.66650.4520.4180.4350.3512.50.2270.2190.2230.1391.250.1840.1880.1860.10200.0850.0830.084 | ||||||
货期：3-5 working days
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