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产品描述：人丝氨酸蛋白酶抑制剂B5(SERPINB5) 酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SERPINB5含量。SERPINB5 是重要靶点。它是丝氨酸蛋白酶抑制剂，在多种生理过程中发挥作用。研究发现其表达异常与肿瘤发生发展有关，能抑制肿瘤细胞迁移、侵袭等，还参与调控细胞凋亡，有望成为肿瘤诊断和治疗的潜在生物标志物及靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于肿瘤发生机制研究、药物靶点筛选、肿瘤微环境分析等科研领域，为探索SERPINB5在疾病进程中的分子调控网络及作为潜在生物标志物的应用价值提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Maspin ELISA Kit; Ovalbumin ELISA Kit; Peptidase inhibitor 5 ELISA Kit; PI-5 ELISA Kit; PI5 ELISA Kit; protease inhibitor 5 (maspin) ELISA Kit; Protease inhibitor 5 ELISA Kit; Serine (or cysteine) peptidase inhibitor; clade B; member 5 ELISA Kit; serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member 5 ELISA Kit; Serpin B5 ELISA Kit; serpin family B member 5 ELISA Kit; Serpin peptidase inhibitor ELISA Kit; Serpin peptidase inhibitor; clade B (ovalbumin); member 5 ELISA Kit; Serpinb5 ELISA Kit; SPB5_HUMAN ELISA Kit; Spi5 ELISA Kit; Spi7 ELISA Kit
缩写：SERPINB5
Uniprot No.：P36952
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINB5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-93 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 97-102 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-100 | |||||
回收率：
| The recovery of human SERPINB5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.2862.2262.2562.07451.8761.8461.8611.6792.51.5621.5501.5561.3741.251.1571.1421.1500.9680.6250.7180.7290.7240.5420.3120.4800.4980.4890.3070.1560.3180.3240.3210.13900.1810.1830.182 | ||||||
货期：3-5 working days
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