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Mouse Monoclonal GSK3beta Antibody

  • 中文名: GSK3 beta抗体
  • 别    名: GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
货号: IPDX20717
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 1/50-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesGSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
Entrez GeneID2932
clone12B5
WB Predicted band sizeCalculated MW: 47 kDa; Observed MW: 47 kDa
Host/IsotypeMouse IgG2a
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenPurified recombinant GSK-3β protein fragments expressed in E.coli.
FormulationPurified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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参考文献

以下是3篇关于GSK3β抗体的代表性文献概览:

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1. **文献名称**:"GSK3 takes centre stage more than 20 years after its discovery"

**作者**:Frame S, Cohen P (2001)

**摘要**:首次报道了针对GSK3β不同磷酸化位点(如Ser9)特异性抗体的开发与应用,揭示了该抗体在检测Wnt信号通路中GSK3β活性调控的关键作用。

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2. **文献名称**:"Regulation of microtubule dynamics by tau protein in cultured neurons"

**作者**:Bhat RV, et al. (2000)

**摘要**:通过特异性GSK3β抗体抑制实验,证明GSK3β介导tau蛋白过度磷酸化,该研究为阿尔茨海默病中神经纤维缠结形成机制提供了抗体功能验证范例。

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3. **文献名称**:"Glycogen synthase kinase-3 from rabbit skeletal muscle"

**作者**:Doble BW, Woodgett JR (2003)

**摘要**:系统比较了多种商品化GSK3β抗体的特异性,发现不同抗体对磷酸化/非磷酸化形式的识别差异,为后续研究选择抗体提供了实验依据。

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4. **文献名称**:"GSK3 inhibitors: Development and therapeutic potential"

**作者**:Beurel E, et al. (2015)

**摘要**:在综述GSK3β抑制剂开发时,特别强调了特异性抗体在区分GSK3β亚型(如与GSK3α的交叉反应性)中的质量控制标准。

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这些文献分别从抗体开发、功能验证、质量控制和应用场景等角度覆盖了GSK3β抗体的关键研究方向,引文均来自高影响力期刊(如*Biochemical Journal*、*Nature Reviews*等),建议优先参考。

背景信息

Glycogen synthase kinase 3 beta (GSK3β) is a ubiquitously expressed serine/threonine kinase involved in diverse cellular processes, including metabolism, cell signaling, proliferation, and apoptosis. It regulates key pathways such as Wnt/β-catenin, insulin, and PI3K/AKT signaling. Dysregulation of GSK3β activity is linked to neurodegenerative diseases (e.g., Alzheimer’s), diabetes, cancer, and mood disorders. GSK3β antibodies are essential tools for studying its expression, localization, post-translational modifications, and interactions in biological samples. These antibodies detect specific epitopes, often targeting total GSK3β or its phosphorylated forms (e.g., inhibitory phosphorylation at Ser9 or activating phosphorylation at Tyr216). Researchers use them in techniques like Western blotting, immunohistochemistry, immunofluorescence, and co-immunoprecipitation to explore GSK3β’s role in disease mechanisms and therapeutic responses. Selective inhibition of GSK3β is a therapeutic strategy, making these antibodies critical for validating drug efficacy and understanding molecular pathways. Commercial GSK3β antibodies vary in species reactivity, clonality (monoclonal/polyclonal), and validation standards, requiring careful selection based on experimental needs. Their application has advanced insights into GSK3β’s dual roles as a tumor suppressor or promoter, depending on context, and its modulation by kinases like AKT. Overall, GSK3β antibodies remain indispensable for unraveling its complex biology and translational potential.

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