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Human Antithrombin III(AT III)ELISA Kit

  • 中文名： 人抗凝血酶III(AT III)ELISA Kit
货号: IPD-E17902h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人抗凝血酶III(AT III)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SERPINC1含量。SERPINC1 是重要的靶点。它是一种丝氨酸蛋白酶抑制剂，参与凝血调控。深入研究其作用机制，有助于了解凝血相关疾病的发生发展过程，为寻找新的治疗靶点和开发治疗凝血异常的药物等提供理论基础。试剂盒检测范围为0.82 ng/mL-600 ng/mL，适用于心血管疾病模型研究、凝血机制探索、药物开发中的抗凝活性评估等科研场景，为体外实验提供灵敏可靠的分析工具。实验操作标准化，支持多样本类型检测，满足基础医学和生命科学领域对凝血系统调控机制的研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    ANT3_HUMAN ELISA Kit; Antithrombin ELISA Kit; Antithrombin III ELISA Kit; Antithrombin-III ELISA Kit; AntithrombinIII ELISA Kit; AT 3 ELISA Kit; AT III ELISA Kit; AT3 ELISA Kit; AT3D ELISA Kit; ATIII ELISA Kit; Heparin cofactor I ELISA Kit; MGC22579 ELISA Kit; Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 ELISA Kit; Serine cysteine proteinase inhibitor clade C member 1 ELISA Kit; Serine proteinase inhibitor clade C member 1 ELISA Kit; Serpin C1 ELISA Kit; Serpin family C member 1 ELISA Kit; Serpin peptidase inhibitor clade C (antithrombin) member 1 ELISA Kit; SERPINC1 ELISA Kit; THPH7 ELISA Kit
  • 缩写：
    SERPINC1
  • Uniprot No.：
    P01008
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.82 ng/mL-600 ng/mL
  • 灵敏度：
    0.82 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human AT III in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1000

    Average %

    103


    Range %

    99-109


    1:2000

    Average %

    101


    Range %

    97-106


    1:4000

    Average %

    99


    Range %

    92-106


    1:8000

    Average %

    108


    Range %

    101-115


  • 回收率：

    The recovery of human AT III spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    96

    91-103


    EDTA plasma (n=4)

    101

    94-108


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    600

    2.632

    2.624

    2.628

    2.426


    200

    2.272

    2.167

    2.220

    2.017


    66.67

    1.677

    1.728

    1.702

    1.500


    22.22

    1.055

    1.029

    1.042

    0.840


    7.4

    0.856

    0.821

    0.839

    0.636


    2.47

    0.526

    0.553

    0.540

    0.338


    0.82

    0.307

    0.294

    0.300

    0.098


    0

    0.199

    0.205

    0.202




  • 货期：
    3-5 working days

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